The 64th JSAP Spring Meeting, 2017
Mar 14 - Mar 17, 2017PACIFICO YOKOHAMA
The 64th JSAP Spring Meeting, 2017
Mar 14 - Mar 17, 2017PACIFICO YOKOHAMA
[16a-514-5]Single Cell Receptor Protein Analysis using Centrifugal Microfluidic Device
〇Takaaki Okajima1, Wilfred Espulgar1, Hyota Takamatsu2, Wataru Aoki3, Masato Saito1, Atsushi Kumanogoh2, Mitsuyoshi Ueda3, Eiichi Tamiya1(1.Osaka Univ., 2.Osaka University, 3.Kyoto Univ.)
Keywords:
single cell,microfluidic device,receptor protein analysis
Receptor proteins on cell membranes are involved in important cell processes such as differentiation and metabolism of cells, and analysis of their expression state is important. The goal of this study is to develop a technology to comprehensively analyze the receptor protein expressed on a single cell. We developed a microfluidic device that captures cells by centrifugal rotation. We constructed a system that repeatedly analyzes the expressed receptor of single cell using receptor analysis with fluorescence-labeled antibodies and photo bleaching.