The 79th JSAP Autumn Meeting, 2018
Sep 18 - Sep 21, 2018Nagoya Congress Center
The 79th JSAP Autumn Meeting, 2018
Sep 18 - Sep 21, 2018Nagoya Congress Center
[19a-212B-7]Detection sensitivity based on probe immobilization amount in DNA microarray
〇Yuki Miyauchi1, Takashi Tadenuma1, Tomoyuki Taguchi1, Takeo Tanaami1(1.Yokogawa)
Keywords:
DNA microarray,FRET,fluorescence
A FRET probe immobilized on a substrate is used for a DNA microarray. The FRET probe consists of a pair of a fluorescent molecule modified probe and a quenching molecule modified probe. The two probes are partially linked with sequences complementary to each other. This FRET probe is quenched in the closed state. When the target DNA is hybridized, quenching is released and fluorescence is detected. We have confirmed the light intensity change in the quenching state and the light emitting state depending on the concentration of the FRET probe immobilized on the substrate.