JVSS 2021
Nov 3 - Nov 5, 2021Online
JVSS 2021
Nov 3 - Nov 5, 2021Online
[1Da03]Development of a CNT tip for internal chromosome measurement by 3D-AFM
*Keigo Teramae1, Keisuke Miyazawa1,2, Ryohei Kojima1, Kaori Hirahara3, Shin-Ichi Horike4, Takeshi Fukuma1,2(1. Graduate School of Natural Science and Technology, Kanazawa University, 2. WPI-NanoLSI, Kanazawa University, 3. Graduate School of Engineering, Osaka University, 4. Research Center for Experimental Modeling of Human Disease, Kanazawa University)
Three-dimensional atomic force microscopy is a technique that has a capable of visualizing three-dimensional fluctuating molecular structures at the solid-liquid interface of a sample. In the biological and medical fields, this 3D-AFM is expected to be used to measure the 3D internal structure of three-dimensional biological samples such as cells and chromosomes. In this study, we fabricated sharp probes made of carbon nanotubes (CNTs) using an electron microscope and performed internal measurements of chromosomes. As a result, the CNT tip penetrated into the chromosome, and it is highly likely that the force distribution reflecting the internal information was obtained. Furthermore, this method will be expected to apply to a wide variety of biological samples, not only chromosomes.