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Wed. Dec 3, 2025

Thu. Dec 4, 2025

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Development and optimization of gene knock-in technology with the elimination of a large genomic fragment using CRISPR-Cas3 in human cultured cells

Demand for gene knock-in technology, which accurately inserts a DNA fragment into a target gene locus, is extremely high, necessitating the development and improvement of methods that can perform this process simply and efficiently. This symposium will introduce a variety of approaches and related technologies for gene knock-in, from classical methods to the latest technologies. The speakers will discuss advantages, challenges and its application of the technology, as well as future prospects of each method.

【J】The new trend in knock-in technology that is expanding from CRISPR-Cas9

The 48th Annual Meeting of the Molecular Biology Society of Japan

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[1PS-04-04(1P-016)]Development and optimization of gene knock-in technology with the elimination of a large genomic fragment using CRISPR-Cas3 in human cultured cells

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