Stem cells and advanced tissue engineering for regenerative medicine
2021年1月25日〜1月28日WEB
Stem cells and advanced tissue engineering for regenerative medicine
2021年1月25日〜1月28日WEB
[13]“Click” Hydrogels: past, present, and mostly future
○Vianney Delplace(Post-doctoral Fellow, Université de Nantes, Oniris, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229)
Education:
In 2011, Vianney Delplace completed a master’s degree in chemical engineering from ESCOM, in parallel with a cross-disciplinary master of science from Chimie ParisTech and Sorbonne University. He then joined the team of Prof. Patrick Couvreur, at Institut Galien Paris-Sud (CNRS 8612), and completed a PhD in polymer science and nanomedicine, with a thesis dedicated to the development of innovative synthetic strategies for the design of biodegradable and biofunctional vinyl polymers. In 2015, he joined the team of Prof. Molly Shoichet, at the University of Toronto, as a post-doctoral fellow, where he developed a variety of hydrogel-based systems for the investigation and treatment of retinal degenerations. Member of the RMeS Lab (INSERM 1229) in Nantes since 2018, his current research focuses on the design of injectable synthetic extracellular matrices for 3D cell culture, bioprinting and material-assisted cell therapy.
Hydrogel design is a booming field of research. The last ten years have seen the development of many new crosslinking strategies tailored to address specific roadblocks to further advance biomedical applications, in particular 3D cell culture and material-assisted cell therapy. Yet, most of them require external stimuli or catalysts, are not entirely bioorthogonal, or have inherent limitations (e.g., limited stability, slow gelation rate). Thus, hydrogels that would be fully tunable, fast-gelling, biocompatible and, yet, easy to synthesize and use, remain to be designed. In this context, innovative “click” and bioorthogonal reactions are being explored.
In this presentation, biomaterial challenges and design criteria related to cell encapsulation for various biomedical applications will be discussed. The concept of “click” chemistry will be introduced, and a critical state-of-the-art of the existing “click” crosslinking strategies will be presented. Focusing on polysaccharide-based hydrogels, I will then present our most recent work on universally applicable network platforms and their potential applications, including the use of the inverse electron-demand Diels-Alder (IEDDA) reaction, the strain-promoted azide-alkyne cycloaddition (SPAAC) and novel dynamic covalent networks.
Using hyaluronic acid as a polymer of interest, I will demonstrate how we successfully synthesized a variety of hydrogel precursors in single-step reactions from commercially available compounds, and how these precursors form hydrogels upon simple mixing under physiological conditions. I will then present a roadmap for the physicochemical characterization and optimization of hydrogels and how it allowed us to design gels that are minimally-swelling, fast-forming, and cytocompatible, with stiffness tunable over orders of magnitude. Various applications of these “click” hydrogels, spanning from explant two-photon imaging to bioprinting, will be presented as a demonstration of their versatility.
Finally, new concepts in hydrogel design for advanced biomimicry, such as programmable hydrogels and peptide/protein patterning, will be introduced, paving the way toward new generations of hydrogels to come.
ACKNOWLEDGEMENTS: the author thanks the Fondation pour la Recherche Médicale (ARF201809007012), the Nantes Excellence Trajectory program (NExT Junior Talent 2018), and the Marie Skłodowska-Curie Actions program (MSCA-IF-RI 2019), for their financial support. Program [PDF]
In this presentation, biomaterial challenges and design criteria related to cell encapsulation for various biomedical applications will be discussed. The concept of “click” chemistry will be introduced, and a critical state-of-the-art of the existing “click” crosslinking strategies will be presented. Focusing on polysaccharide-based hydrogels, I will then present our most recent work on universally applicable network platforms and their potential applications, including the use of the inverse electron-demand Diels-Alder (IEDDA) reaction, the strain-promoted azide-alkyne cycloaddition (SPAAC) and novel dynamic covalent networks.
Using hyaluronic acid as a polymer of interest, I will demonstrate how we successfully synthesized a variety of hydrogel precursors in single-step reactions from commercially available compounds, and how these precursors form hydrogels upon simple mixing under physiological conditions. I will then present a roadmap for the physicochemical characterization and optimization of hydrogels and how it allowed us to design gels that are minimally-swelling, fast-forming, and cytocompatible, with stiffness tunable over orders of magnitude. Various applications of these “click” hydrogels, spanning from explant two-photon imaging to bioprinting, will be presented as a demonstration of their versatility.
Finally, new concepts in hydrogel design for advanced biomimicry, such as programmable hydrogels and peptide/protein patterning, will be introduced, paving the way toward new generations of hydrogels to come.
ACKNOWLEDGEMENTS: the author thanks the Fondation pour la Recherche Médicale (ARF201809007012), the Nantes Excellence Trajectory program (NExT Junior Talent 2018), and the Marie Skłodowska-Curie Actions program (MSCA-IF-RI 2019), for their financial support. Program [PDF]