Stem cells and advanced tissue engineering for regenerative medicine
2021年1月25日〜1月28日WEB
Stem cells and advanced tissue engineering for regenerative medicine
2021年1月25日〜1月28日WEB
[14]Generation of mouse and human intestinal progenitor cells using direct reprogramming technology.
○Shizuka Miura, Kenichi Horisawa, Atsushi Suzuki(Specially appointed assistant professor, Medical Institute of Bioregulation, Kyushu University)
Contact:
E-mail: smiura@bioreg.kyushu-u.ac.jp
E-mail: smiura@bioreg.kyushu-u.ac.jp
Education:
2018.3 Ph.D., in Medicine, Kyushu University
Professional Career:
2018.4-present Specially appointed assistant professor, Medical Institute of Bioregulation, Kyushu University
Recent studies have demonstrated establishment of culture systems about adult tissue stem cells. Particularly, intestinal stem cells were difficult to maintain for a long term in vitro, but it became possible to maintain them by using Matrigel culture system. However, it is difficult to collect the intestinal tissue from patients. So, it is expected to develop the less invasive method. Meanwhile, recently, direct reprogramming technology enables us to convert into the other types of cells from the differentiated cells. For example, myoblasts, neurons and hepatocytes can be generated directly from fibroblasts using this technology. In our previous study, we have succeeded to generate induced fetal intestine-derived progenitor cells (iFIPCs) from mouse embryonic fibroblasts and human umbilical vein endothelial cells. Similar to FIPCs, iFIPCs can form spherical organoids (SOs), and iFIPC-derived SOs can develop into budding organoids (BOs). iFIPC-derived BOs contain cells with the properties of ISCs, designated as induced ISCs (iISCs), which have self-renewal capacity and multi-lineage differentiation potential. Upon transplantation into the injured colon, iFIPC-derived SOs functionally reconstitute colonic epithelial tissues. Therefore, iFIPCs may be useful in the study of intestinal development and applications toward drug screening and regenerative therapy. Program [PDF]