Stem cells and advanced tissue engineering for regenerative medicine
2021年1月25日〜1月28日WEB
Stem cells and advanced tissue engineering for regenerative medicine
2021年1月25日〜1月28日WEB
[6]Generating human innervated intestinal tissue from pluripotent stem cells.
○Maxime M. Mahe(Associate Professor, INSERM TENS, UMR 1235)
Education:
In 2012, Dr. Maxime Mahe obtained his PhD from the University of Nantes in Neurogastroenterology. In 2012, he joined the Division of Pediatric General and Thoracic Surgery at CCHMC, under the leadership of Michael Helmrath, MD, MS and Jim Wells, PhD, and worked on developing new methodologies for the study of murine and human intestinal stem cells. The aim of my research was to develop and use intestinal stem cell culture techniques to study the mechanisms that result in regional specific intestinal stem cell patterning. With the goal of studying regional patterning in the small intestine, they were the first group to successfully generate 3-dimensional intestinal human PSCs and show functional maturation following engraftment into mice. In 2017, Dr. Mahe has been recruited as an assistant professor (Inserm CRCN) to establish a research program on the effects of the enteric nervous system on intestinal development using innovative approaches.
The use of pluripotent stem cells offers great avenues to generate human tissues. The understanding of intestinal development and its translation to human pluripotent stem cells, had allow the field to move forward in understanding intestinal development and gastrointestinal diseases. In this talk, I will highlight our previous work which had focused on generating functional human intestinal organoids (HIOs) from embryonic stem cells and induced pluripotent stem cells. Building on this model, I will highlight the additional complexity we were able to engineer in order to gain insights into intestinal physiology and diseases. In this context, the development of human intestine with an enteric nervous system (ENS) represents a real opportunity to expand our knowledge into the effect of ENS on intestinal development and toward the understanding of pathophysiological processes leading to functional gastrointestinal neuropathies. Finally, I will delineate the forthcoming strategies that could be used to create a fully functional intestine for intestinal regenerative medicine. Program [PDF]