Presentation Information
[1P-049]Metal-dependent activity regulation of a compact-sized DNAzyme containing an imidazole-4-carboxylate base pair
*Lingyun Hu1, Yusuke Takezawa1, Takahiro Nakama1, Mitsuhiko Shionoya1 (1. The University of Tokyo)
Keywords:
DNA,DNAzyme,Cu(II),Imidazole-4-carboxylate,Artificial base pair
DNAzymes are functional DNA molecules that catalyze certain chemical reactions. The 8-17 DNAzyme stands out due to its compact size and high catalysis capability. However, the compact-sized structure makes it difficult to receive modifications without affecting its activity. Herein, we developed a Cu(II)-responsive DNAzyme by incorporating a previously known Cu(II)-mediated imidazole-4-carboxylate base pair (ImC–Cu(II)–ImC) into the short stem region of 8-17 DNAzyme. The activity of the modified DNAzyme was increased 4.7-fold when an equimolar Cu(II) ion was added, showing evident metal-responsive regulation of the activity. These results pave the road for further development of metal-responsive DNA devices.