Presentation Information
[1P-059]Enhanced Stability and Structural Changes of DNA Origami by Enzymatic Ligation
*Kirankumar Krishnamurthy1, Arivazhagan Rajendran1, Eiji Nakata1, Takashi Morii1 (1. Institute of Advanced Energy, Kyoto University)
Keywords:
DNA nanotechnology,Enzymatic ligation
The structural DNA nanotechnology has attracted much attention over the last decade due to the addition of the scaffolded DNA origami method to the field. One major issue is its stability that prevents them from applications in a wide range of conditions to withstand the thermal, mechanical, and chemical modifications. Most of the DNA origami structures melt below 60°C due to the presence of discontinuities in the phosphate backbone of the staple strands. In this study, a detailed analysis and optimized conditions for the enzymatic ligation of the staple strands in 4 types of 2D DNA origami is reported.