The 15th Symposium on Biorelevant Chemistry
Sep 8 - Sep 10, 2021Held Online
The 15th Symposium on Biorelevant Chemistry
Sep 8 - Sep 10, 2021Held Online
[3A-05]Improvement of Neutralization Activity for SARS-CoV-2 and Variants of Concerns by Affinity Maturation of Monobody.
*Taishi Kondo1, Kazuhiro Matsuoka2, Tomoshige Fujino1, Shun Umemoto1, Gosuke Hayashi1, Yasumasa Iwatani2,3, Hiroshi Murakami1,4(1. Department of Biomolecular Engineering, Graduate School of Engineering, Naoya University, 2. Department of Infectious Diseases and Immunology, Clinical Research Center, National Hospital Organization Nagoya Medical Center, 3. Division of Basic Medicine, Graduate School of Medicine, Nagoya University, 4. Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University)
Keywords:
Molecular Evolution Engineering,Antibody-Like Proteins,SARS-CoV-2,Neutralizing Antibody
これまでに、迅速な人工抗体製法TRAP提示法を用いて、SARS-COV-2スパイクタンパク質に対する人工抗体Monobodyの創製に成功している。そして、得られたMonobodyをもとに飽和変異ライブラリを構築し、再度セレクションを行うことで親和性と中和活性の大幅な向上を達成したことについて報告する。