The 16th Symposium on Biorelevant Chemistry
Sep 10 - Sep 12, 2022Held On-site and Online
The 16th Symposium on Biorelevant Chemistry
Sep 10 - Sep 12, 2022Held On-site and Online
[1B-10]Monobodies with neutralizing activity against SARS-CoV-2 Omicron
*Taishi Kondo1, Kazuhiro Matsuoka2, Tomoshige Fujino1, Shun Umemoto1, Gosuke Hayashi1, Yasumasa Iwatani2,3, Hiroshi Murakami1,4(1. Department of Biomolecular Engineering, Graduate School of Engineering, Naoya University, 2. Department of Infectious Diseases and Immunology, Clinical Research Center, National Hospital Organization Nagoya Medical Center, 3. Division of Basic Medicine, Graduate School of Medicine, Nagoya University, 4. Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University)
Keywords:
Molecular Evolution Engineering,Antibody-Like Proteins,SARS-CoV-2,Neutralizing Antibody
新たに出現したOmicron株は、多くの抗体医薬品から逃避するため、有効な中和抗体の開発が重要である。TRAP提示法を用いて新たにセレクションを行ない、解離定数がOmicron RBDとBA.2 RBDについてKD = 0.61, 0.74 nMと強く結合し、Omicron株とOmicron BA.2株それぞれをIC50 = 0.41, 0.49 nM で感染を阻害するクローンの取得に成功した。