Presentation Information
[P172]In situ detection of point mutation in tumor cell block specimens by monitoring single-molecule fluorescence blinking
*Shuya FAN1, Jie XU1, Yasuko OSAKADA1,2, Katsunori HASHIMOTO3, Kazuya TAKAYAMA4, Atsushi NATSUME4,5,6, Masaki HIRANO4,7, Atsushi MARUYAMA8, Mamoru FUJITSUKA1, Kumi KAWAI9, Kiyohiko KAWAI1 (1. SANKEN, Osaka University, 2. IACS, Osaka University, 3. Dept. of Med. Sci., Shubun University, 4. Craif. Inc., 5. Institute of Innovation for Future Society, Nagoya University, 6. Dept. of Neurosurgery, Kawamura Medical Associate Hospital, 7. Div. of Molecular Oncology, Aichi Cancer Center Research Institute, 8. Dept. of Life Sci. and Technol, Tokyo Institute of Technology, 9. Dept. of Health and Nutrition, Shubun University)
Keywords:
Single-molecule detection,Fluorescence blinking,Point mutation detection
Mismatches in nucleic acids could be sensitively detected by measuring electron transfer rate. Herein, we achieved point mutation detection both in synthetic RNA and tumor cell block specimens by monitoring single-molecule fluorescence blinking.