Presentation Information
[P186]Chemically Enhanced Stability of 2D and 3D DNA Origami Nanostructures
*KiranKumar Krishnamurthy1, Arivazhagan Rajendran1, Eiji Nakata1, Takashi Morii1 (1. Institute of Advanced Energy, Kyoto University)
Keywords:
DNA nanotechnology,Enzymatic ligation,Chemical ligation,DNA origami
The scaffolded DNA origami method has gained much attention for its versatility in designing complex nano-architectures and have made them novel scaffolds in various biochemical applications. Regardless of the advantages, they exhibit low stability to undergo any further modifications required for various applications. This is mainly due to discontinuities (nicks) in the phosphate backbone of the staple strands in the DNA origami. Though enzymatic ligation is commonly used in molecular biology to seal the nicks in the duplex DNA, their use is limited and less characterized. The alternate way is to ligate them by chemical methods. Here we discuss two such methods that offer faster reaction rates and higher ligation yield.