The 17th Symposium on Biorelevant Chemistry
Sep 8 - Sep 10, 2023Tokyo University of Science
The 17th Symposium on Biorelevant Chemistry
Sep 8 - Sep 10, 2023Tokyo University of Science
[1OB-06]Development of PureCap Method That Enables Synthesis of Fully Capped mRNA and Evaluation of Biological Activities
*Masahito Inagaki1, Naoko Abe1, Li Zhenmin1, Yuko Nakashima2, Acharyya Susit1, Meng Zheyu1, Mizuki Tada1, Tatsuma Ishida1, Pingxue Lyu1, Fumitaka Hashiya2, Yasuaki Kimura1, Satoshi Uchida3,4, Hiroshi Abe1,5,6(1. Nagoya University, 2. Research Center for Materials Science, Nagoya University, 3. Medical Research Institute, Tokyo Medical and Dental University, 4. Innovative Center of NanoMedicine (iCONM) , 5. JST-CREST , 6. Institute for Glyco-core Research (iGCORE) )
Keywords:
Messenger RNA,Cap Structure,PureCap Method,PureCap Analogs,Photo-Removable Hydrophobic Protecting Group,Reverse-Phase High-Performance Liquid Chromatography
PureCap法と名付けた新規mRNA合成技術を開発し、世界初の完全キャップ化mRNAの製造を実現した。PureCap法により合成した超高純度mRNAは既存の合成法により製造したmRNAよりも、モデルマウスを用いたin vivo実験により7倍以上高い翻訳活性を示し、PureCap法の有用性の実証に成功した。