The 17th Symposium on Biorelevant Chemistry
Sep 8 - Sep 10, 2023Tokyo University of Science
The 17th Symposium on Biorelevant Chemistry
Sep 8 - Sep 10, 2023Tokyo University of Science
[1OC-09]Application of prediction parameters for RNA/DNA hybrid duplex stability in the advancement of therapeutics
*Dipanwita Banerjee1, Hisae Tateishi-Karimata1, Saptarshi Ghosh1, Tatsuya Ohyama1, Tamaki Endoh1, Shuntaro Takahashi1, Naoki Sugimoto1,2(1. FIBER, Konan University, 2. FIRST, Konan University)
Keywords:
RNA/DNA hybrid duplex,cellular condition,stability prediction,CRISPR-Cas9,ASO
RNA/DNA hybrid duplexes form everywhere in living cells. The hybrid duplex regulates important biological reactions such as replication and transcription and also therapeutic techniques like gene silencing ASO (Antisense Oligonucleotide) and gene editing CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9. Thus, the stability prediction for hybrid duplex in cellular conditions is essential for understanding the biological reactions and developing therapeutic techniques in cell. We derived new parameters under diverse molecular crowding conditions that predicted accurately the stability of hybrid duplex under diverse in vitro and in cell conditions. Among all applications, we displayed the use of our new parameters to improve therapeutic efficiency. For example, our derived parameters can estimate knockout efficiency of CRISPR-Cas9. The estimation of CRISPR efficiency for different target sites will help to shorten length of guide RNA, which will be also useful for improving target specificity by decreasing off-target binding probability.