Presentation Information
[2PB-47]Development of a multiple-target detectable sensor based on DNA nanostructure
*Mashal Asif1, Eiji Nakata1, Khongurzul Gerelbataar1, Yuya Shibano1, Takashi Morii1 (1. Institute of Advanced Energy, Kyoto University)
Keywords:
ratiometric fluorescent probe,pH sensitive fluorophore,DNA nanostructure,DNA origami scaffold,pH detection
This study aims to develop an innovative device for accurate detection of intracellular pH and other parameters using fluorescent probes on DNA nanostructures. Assembling multiple sensors on a DNA scaffold offers precise spatial organization and programmability. Ratiometric detection using pH-sensitive (CF) and pH-insensitive (CR) fluorophores on the DNA nanostructure enables varied responses to pH changes. The pH detection range is expanded by incorporating sensors with different pKa values, like Oregon Green (OG). This approach can be extended to other targets, such as enzyme activity, using appropriate fluorescent probes. The presentation will discuss progress in this direction.