[1S00a] 01. ASBA - Part 2

2:00 PM - 2:20 PM JST(5:00 AM - 5:20 AM UTC)

[1ASBA-07-KL] Synthetic Biology of Acetogens for Sustainable C1 gas bioconversion

○Byung Kwan Cho¹ (1. KAIST (Korea))

Keywords:

Acetogen,C1 gas biorefinery,Synthetic metabolic pathway,Adaptive laboratory evolution

The C1 gases generated in various industrial processes mainly consist of carbon dioxide (CO₂) and carbon monoxide (CO). Acetogens have emerged as biocatalysts for recycling C1 gases by converting them into value-added biochemicals using the Wood-Ljungdahl (WL) pathway, the most efficient natural CO₂-fixation pathway. However, despite the advantages of using acetogens as biocatalysts, most previous studies have focused on understanding the biochemistry of the WL pathway and the physiological changes in several model acetogen strains, due to limited knowledge of the genetic landscape under C1-autotrophic conditions. To overcome those limitations, we have focused on understanding the C1 fixation mechanism in acetogens at the molecular level using systems biology. Next, using genetic information, we constructed several synthetic biology-based genetic bio-parts and modules for controlling metabolic flux in acetogens. Finally, to develop highly efficient C1 conversion biocatalysts, we introduced several value-added biochemical pathways into the acetogen and produced those chemicals using C1 gas fermentation.