[1S00a] 01. ASBA - Part 2

2:20 PM - 2:35 PM JST(5:20 AM - 5:35 AM UTC)

[1ASBA-08] A Modular Synthetic Biology Approach to Soil Bioremediation: Restoring Degraded Ecosystems through Multi-functional Microbial Networks

○Sangrak Jin¹ (1. Yeungnam University (Korea))

Keywords:

Soil microbiome,Artificial Consortium,Synthetic Biology,Bioremediation,Adaptive Laboratory Evolution

Soil microbiomes play a critical role in maintaining ecosystem functions, driving nutrient cycling, and buffering plants against environmental stressors. However, anthropogenic disturbances and natural disasters, such as wildfires, severely degrade these microbial networks and introduce toxic post-fire byproducts. This study aims to develop a synthetic microbial consortium designed to restore soil health and enhance plant productivity in degraded landscapes. The proposed consortium integrates five specialized taxa with complementary roles: Pseudomonas putida for the detoxification of wildfire-derived pollutants and community control; Pseudomonas simiae and Bacillus subtilis for rhizosphere colonization, growth promotion, and consortium stability; Rhizobium leguminosarum for biological nitrogen fixation; and Streptomyces spp. for biocontrol against soil-borne pathogens. Current research focuses on the foundational optimization of P. putida. We are employing adaptive laboratory evolution (ALE) to enhance its resilience and degradative capacity toward post-fire toxins. Simultaneously, we are screening high-performance genetic bioparts and investigating quorum-sensing mechanisms to ensure precise spatial organization and coordinated metabolic flux within the rhizosphere. This modular synthetic biology approach provides a robust strategy for soil bioremediation and sustainable agricultural restoration in post-fire environments.