[1S00e] 01. ASBA - Part 3

5:05 PM - 5:20 PM JST(8:05 AM - 8:20 AM UTC)

[1ASBA-14] Reprogramming Yarrowia lipolytica for Adipic Acid Production via Plant-derived Acyl-CoA Oxidase Integration

○Seung-Ho Baek^1,2 (1. Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology (Korea), 2. Advanced Materials and Chemical Engineering, University of Science & Technology (Korea))

Keywords:

Bio adipic acid,Yarrowia lipolytica,Metabolic engineering,Synthetic biology

The demand for adipic acid is increasing, however, its chemical production poses a significant environmental issue. Here, we present an efficient microbial production method to produce bio-based adipic acid from renewable resources using oleaginous yeast Yarrowia lipolytica. We first engineered a strain by introducing the plant-derived acyl-CoA oxidase gene Arabidopsis thaliana ACX3 (At.ACX3) to convert methyl laurate into the desired C6 chain length. The strain expressing At.ACX3 outperformed the POX1 and POX3 deletion strain, which had highest adipic acid production among the deletion strains. To further enhance production, we modified ω-oxidation genes. Adding an extra copy of the fatty alcohol oxidase gene (FAO1) significantly increased production. We then examined three cytochrome P450 enzymes (ALK) with NADPH-cytochrome P450 reductases (CPR1), finding that ALK5 and CPR1 overexpressing strains achieved a five-fold increase. Finally, integrating extra copies of CPR1, ALK5, and FAO1 further improved production level. While microbial production is not yet a viable alternative to petrochemical methods, this study demonstrates its potential for a future alternative.