Presentation Information
[1AFOB-21]Development of a Sustainable Recovery Platform for Rare Earth Elements Utilizing Genetically Engineered M13 Bacteriophage
○Chia-Yu Chang1, Hong-Zhen He1, Wei-Chi Ho1, Shen-Long Tsai1 (1. Department of Chemical Engineering, National Taiwan University of Science and Technology (Taiwan))
Keywords:
Rare earth element,Phage display,REE binding peptide
Rare earth elements (REEs) are important strategic resources because of their unique electromagnetic and luminescent properties, which are essential to high-tech industries and renewable energy technologies. However, their physicochemical similarity makes selective separation challenging. Hence, conventional extraction and separation methods for REEs typically require high energy consumption and lead to significant environmental impacts.
In this study, we aim to develop a bioaffinity-based REE recovery system. Engineered M13 bacteriophages were decorated with lanthanide-binding tag (LBT) peptides on its pVIII major coat protein, allowing for the selective binding of REEs. Cerium ions (Ce3+) served as a model to assess REE adsorption performance of this system. The cerium ion concentrations were measured with inductively coupled plasma optical emission spectrometry (ICP-OES), and the adsorption capacities were calculated appropriately. Selectivity was further validated by comparing with non-REE metal ions (Cu2+ and Zn2+). The results demonstrate that LBT-decorated phage exhibits excellent binding capacity and high selectivity toward REEs. In addition, Langmuir and Freundlich isotherm models, as well as pseudo-first-order and pseudo-second-order kinetic models, were used to investigate the adsorption process.
To facilitate the recovery of REE-loaded phages, a strategy utilizing the SpyTag–SpyCatcher conjugation pair was developed. In this approach, the M13 phage pIII protein was fused with SpyTag, while SpyCatcher was displayed on the yeast surface, resulting in a whole-cell-based recovery system that supports gravity-driven separation.
This study demonstrates a bioaffinity-based REE recovery platform, offering an alternative to traditional extraction methods. Furthermore, this underscores the potential of bioengineering for advanced resource recovery and environmental applications.
In this study, we aim to develop a bioaffinity-based REE recovery system. Engineered M13 bacteriophages were decorated with lanthanide-binding tag (LBT) peptides on its pVIII major coat protein, allowing for the selective binding of REEs. Cerium ions (Ce3+) served as a model to assess REE adsorption performance of this system. The cerium ion concentrations were measured with inductively coupled plasma optical emission spectrometry (ICP-OES), and the adsorption capacities were calculated appropriately. Selectivity was further validated by comparing with non-REE metal ions (Cu2+ and Zn2+). The results demonstrate that LBT-decorated phage exhibits excellent binding capacity and high selectivity toward REEs. In addition, Langmuir and Freundlich isotherm models, as well as pseudo-first-order and pseudo-second-order kinetic models, were used to investigate the adsorption process.
To facilitate the recovery of REE-loaded phages, a strategy utilizing the SpyTag–SpyCatcher conjugation pair was developed. In this approach, the M13 phage pIII protein was fused with SpyTag, while SpyCatcher was displayed on the yeast surface, resulting in a whole-cell-based recovery system that supports gravity-driven separation.
This study demonstrates a bioaffinity-based REE recovery platform, offering an alternative to traditional extraction methods. Furthermore, this underscores the potential of bioengineering for advanced resource recovery and environmental applications.
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