[1S04a] 14. Chemical Biotechnology - Part 2

3:00 PM - 3:15 PM JST(6:00 AM - 6:15 AM UTC)

[1Chemi-10] Cell-Free Synthetic Biology for In Vitro Metabolic Engineering and Biosynthesis

○Jian Li¹ (1. ShanghaiTech University (China))

Keywords:

Cell-free synthetic biology,Biocatalysis,Natural products,High-value chemicals,Chemical biology

The foundational principle of cell-free synthetic biology is that precise, complex biomolecular systems can be constructed without the use of living, intact cells. Because of the absence of cell walls, these open systems allow for easy manipulation, monitoring, sampling, and optimization. Therefore, in vitro cell-free systems have many advantages over in vivo cell systems including high product yields, fast reaction rates, tolerance of toxic precursors and/or products, and highly controlled reaction conditions. Recently, cell-free synthetic systems are becoming important as platforms for enabling biosynthetic routes to proteins, novel biopolymers, and small molecule chemicals. In this context, we take advantage of cell-free biology to design and construct efficient platforms for the rapid and cost-effective next-generation biomanufacturing. Here, I will introduce the construction of in vitro biosynthetic pathways for the synthesis of complex, pharmaceutical natural products such as nonribosomal peptides (NRPs) and ribosomally synthesized and post-translationally modified peptides (RiPPs). Then, I will introduce the use of a cell-free protein synthesis system for constructing artificial enzymatic pathways, which enables one-pot cell-free metabolic engineering and cascade biotransformation.