Presentation Information
[1ACCE-10]Quantitative bio-assessment of nickel using morphological changes of macrophage-like cells
○Akiko Ogawa1, Hikaru Tamaru1 (1. National Institute of Technology (KOSEN), Suzuka College (Japan))
Keywords:
nickel,morphological change,bio-assessment
[Purpose]
Nickel is a well-known causative element of metallic allergy categorized as type IV delayed-type hypersensitivity. Although the detailed mechanism of metallic allergy remains under investigation, nickel ions (Ni2+) are released from material surfaces and penetrate the skin or interact with macrophages and dendritic cells at the early stage, inducing the production and release of inflammatory cytokines. In the present study, we focused on morphological changes of macrophages to develop a quantitative bio-assessment system for evaluating nickel risk.
[Method]
The THP-1 cell line, derived from human monocytes, was used as a macrophage model. Cells were differentiated into macrophage-like cells by stimulation with phorbol 12-myristate 13-acetate (PMA). After 24 h of PMA removal, the differentiated cells were cultured with or without nickel ions at concentrations of 5.01 μM or 50.1 μM for several hours. The cells were observed using phase-contrast microscopy at 0 h, 2 h, and 3.3 h after nickel treatment. The acquired images were divided into smaller regions and categorized into round-shaped cells, spread/flattened cells, and partial cells using individual and region detection modes in the Collie application (ITAGE Co., Ltd.), a no-code image recognition AI platform for teacher model construction.
[Results and Consideration]
The classified images will be further analyzed to quantify morphological categories and evaluate nickel-dependent cellular responses.
Nickel is a well-known causative element of metallic allergy categorized as type IV delayed-type hypersensitivity. Although the detailed mechanism of metallic allergy remains under investigation, nickel ions (Ni2+) are released from material surfaces and penetrate the skin or interact with macrophages and dendritic cells at the early stage, inducing the production and release of inflammatory cytokines. In the present study, we focused on morphological changes of macrophages to develop a quantitative bio-assessment system for evaluating nickel risk.
[Method]
The THP-1 cell line, derived from human monocytes, was used as a macrophage model. Cells were differentiated into macrophage-like cells by stimulation with phorbol 12-myristate 13-acetate (PMA). After 24 h of PMA removal, the differentiated cells were cultured with or without nickel ions at concentrations of 5.01 μM or 50.1 μM for several hours. The cells were observed using phase-contrast microscopy at 0 h, 2 h, and 3.3 h after nickel treatment. The acquired images were divided into smaller regions and categorized into round-shaped cells, spread/flattened cells, and partial cells using individual and region detection modes in the Collie application (ITAGE Co., Ltd.), a no-code image recognition AI platform for teacher model construction.
[Results and Consideration]
The classified images will be further analyzed to quantify morphological categories and evaluate nickel-dependent cellular responses.
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