[1S06m] 15. Advanced Cell and Cell Culture Engineering for Biopharmaceuticals and Functional Food - Part 1

10:00 AM - 10:30 AM JST(1:00 AM - 1:30 AM UTC)

[1ACCE-01-KL] Mammalian Cell and Cell Culture Engineering: A Workhorse of Animal Cell Technology

○Takeshi OMASA¹, Noriko Yamano-Adachi¹ (1. Graduate School of Engineering, The University of Osaka (Japan))

Keywords:

Chinese hamster ovary cells,therapeutic antibody,cell culture engineering

Mammalian cell lines are indispensable platforms for the industrial-scale production of therapeutic antibodies (biologics), owing to their capacity for accurate folding, assembly, and post-translational modifications. Among these mammalian cells, Chinese hamster ovary (CHO) cell lie which was established more than 60 years ago, has emerged as the gold standard for producing therapeutic proteins with high reliability. Recently, we established novel cell lines derived from Chinese hamster (Cricetulus griseus) lung and ovary tissues, broadening the scope of biopharmaceutical manufacturing. These cells were gradually adapted to serum-free conditions by reducing the serum concentration stepwise. The resulting serum-free cell pools were designated as Chinese hamster lung (CHL)-YN (Riken bioresource bank: RCB5004)¹⁾, and Chinese hamster ovary (CHO)-MK²⁾, respectively. Both CHL-YN and CHO-MK cell lines demonstrated rapid proliferation, with doubling times shorter than 10 hours in serum-free chemically defined medium.In this presentation, we will introduce the characteristics of both cell lines and metabolism³⁾ and discuss the impact of these new cell lines on cell/ cell culture engineering in therapeutic antibody manufacturing. Also, we will report the comparative omics profiling reveals differences in biomass, energy production, and vesicle transport between CHO and fast-growing CHL-YN cells⁴⁾.
References
(1)Noriko Yamano-Adachi et al., , Sci. Rep. 10(1):17612 (2020). (2)Kenji Masuda et al. , J. Biosci. Bioeng. 137(6), 471-479 (2024). (3)Puriwat Sukwattananipaat et al., Biotechnol. Bioeng. 121(9), 2848-2867 (2024).
(4)Yu Tsunoda et al., , Sci. Rep. 15(1):39784 (2025).

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