Presentation Information
[2ASBA-14]A Modular Synthetic Chassis: Rational Engineering of Human Adenoviral Scaffolds for Fowl Adenovirus VLP Assembly
○Nurulfiza Binti Mat Isa1,4, Nur Aqilah Binti Akbar Shah1, Norfitriah Binti Mohamed Sohaimi2, Choo Yee Yu1, Mohd Hair Bin Bejo1,3 (1. Laboratory of Vaccine and Biomolecules, Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor (Malaysia), 2. Department of Veterinary Laboratory Diagnostics, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor (Malaysia), 3. Department of Veterinary Pathology & Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor (Malaysia), 4. Department of Cell & Molecular Biology, Faculty of Biotechnology & Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor (Malaysia))
Keywords:
Fowl Adenovirus 8b (FAdV-8b),Inclusion Body Hepatitis (IBH),Virus Like Particles (VLP),Penton Protein,Modular Expression System
[Purpose]
The first FAdV serotype 8b inclusion body hepatitis (IBH) epidemic occurred in Malaysia was in 2005. Traditional chicken vaccines risk viral reversion to virulence and complicate disease surveillance. This study addresses this by employing a recombinant adenoviral vector (rAd) expression system to develop a Virus-Like Particle (VLP) vaccine targeting IBH poultry disease. VLPs eliminate infection risks by lacking genetic material while providing a "virus-like" structure for superior immunity. They enable DIVA strategies (Differentiating Infected from Vaccinated Animals), ensuring poultry exports remain safe and outbreaks are easily monitored.
[Method]
Using a rational engineering approach, we designed a modular expression system where the HAdV vector backbone serves as a heterologous scaffold. We successfully integrated an immunogenic capsid penton protein derived from Aviadenovirus into the HAdV framework. This "plug-and-play" architecture produce high-efficiency "delivery truck" to turn a large population of cells into a VLP factory.
Our "Build-and-Test" phase involved the co-transfection of modified genetic constructs into HEK293T cells. Successful self-assembly of the penton-based VLPs was validated through SDS-PAGE, Western Blotting and Electron Microscopy, confirming that the inter-species protein swap did not compromise the structural integrity of both dodecahedral and icosahedral shell.
[Results]
Electron imaging revealed the presence of regular penton-based VLPs with dodecahedral structures approximately 21 nm in diameter.
[Consideration]
This study demonstrates that the rAd Expression System function as a versatile synthetic scaffold, capable of being "reprogrammed" with avian viral parts. This modular platform not only provides a robust strategy for poultry disease prevention but also advances the SynBio goal of creating customizable macromolecular machines for global health applications.
[Conclusion]
We present a cross-species synthetic biology platform that leverages a human adenovirus chassis for avian vaccine design. By engineering the self-assembly of inter-species viral proteins, we provide a programmable strategy that eliminates the risks of virulence reversion and diagnostic interference. This framework offers a scalable, rapid-response solution to address emerging biosecurity threats in global poultry production.
The first FAdV serotype 8b inclusion body hepatitis (IBH) epidemic occurred in Malaysia was in 2005. Traditional chicken vaccines risk viral reversion to virulence and complicate disease surveillance. This study addresses this by employing a recombinant adenoviral vector (rAd) expression system to develop a Virus-Like Particle (VLP) vaccine targeting IBH poultry disease. VLPs eliminate infection risks by lacking genetic material while providing a "virus-like" structure for superior immunity. They enable DIVA strategies (Differentiating Infected from Vaccinated Animals), ensuring poultry exports remain safe and outbreaks are easily monitored.
[Method]
Using a rational engineering approach, we designed a modular expression system where the HAdV vector backbone serves as a heterologous scaffold. We successfully integrated an immunogenic capsid penton protein derived from Aviadenovirus into the HAdV framework. This "plug-and-play" architecture produce high-efficiency "delivery truck" to turn a large population of cells into a VLP factory.
Our "Build-and-Test" phase involved the co-transfection of modified genetic constructs into HEK293T cells. Successful self-assembly of the penton-based VLPs was validated through SDS-PAGE, Western Blotting and Electron Microscopy, confirming that the inter-species protein swap did not compromise the structural integrity of both dodecahedral and icosahedral shell.
[Results]
Electron imaging revealed the presence of regular penton-based VLPs with dodecahedral structures approximately 21 nm in diameter.
[Consideration]
This study demonstrates that the rAd Expression System function as a versatile synthetic scaffold, capable of being "reprogrammed" with avian viral parts. This modular platform not only provides a robust strategy for poultry disease prevention but also advances the SynBio goal of creating customizable macromolecular machines for global health applications.
[Conclusion]
We present a cross-species synthetic biology platform that leverages a human adenovirus chassis for avian vaccine design. By engineering the self-assembly of inter-species viral proteins, we provide a programmable strategy that eliminates the risks of virulence reversion and diagnostic interference. This framework offers a scalable, rapid-response solution to address emerging biosecurity threats in global poultry production.
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