Presentation Information
[2ASPR-16]Application of EGAO technology to maize and pennycress
〇Miyo Yamane¹, Addie Thompson², Linsey Newton², Daisuke Todaka¹, Motoaki Seki¹ (¹ RIKEN Center for Sustainable Resource Science, Japan, ² Department of Plant, Soil and Microbial Sciences, Michigan State University, USA)
Video Presentation
Keywords:
EGAO technology,ethanol,chemical priming,abiotic stress tolerance,maize,pennycress
Video Presentation
Applying low-concentration ethanol (EtOH) to plants can enhance tolerance to abiotic stresses. We named this approach EGAO technology and aim to promote its global adoption to support stable food production and sustainable agriculture. In this study, we examined the genotype dependence of EtOH responsiveness and its potential impact on nitrogen use efficiency using a genetically diverse maize panel. We also explored the feasibility of applying EGAO technology to pennycress, a cover crop and oilseed species.
We conducted phenotyping of 50 maize lines at field. Target traits included sugar and starch content, SPAD, plant height, canopy reflectance, and grain yield. For each line, we established three treatment groups: nitrogen deficiency, EtOH treatment, and nitrogen application. In maize, EtOH responses varied among lines, suggesting a genotype-dependent effect of EGAO technology. Although variability was substantial and further validation is needed, some lines showed trends consistent with a potential improvement in nitrogen use efficiency under EtOH treatment.
For pennycress, we used the spring type line Spring32-10 and evaluated abiotic stress tolerance following EtOH treatment. Assays are underway, and data collection and analysis are ongoing at the time of abstract submission. The results will be presented at the meeting.
This study indicates that EtOH responsiveness is genotype dependent in maize. This finding motivates future genetic dissection, including genome-wide association studies, to identify loci and candidate genes underlying variation in EGAO responses and to deepen mechanistic understanding of ethanol-mediated priming. Ongoing work in pennycress will test whether EGAO can enhance stress resilience in this cover crop and oilseed, which could help stabilize establishment and expand cultivation in marginal environments.
We conducted phenotyping of 50 maize lines at field. Target traits included sugar and starch content, SPAD, plant height, canopy reflectance, and grain yield. For each line, we established three treatment groups: nitrogen deficiency, EtOH treatment, and nitrogen application. In maize, EtOH responses varied among lines, suggesting a genotype-dependent effect of EGAO technology. Although variability was substantial and further validation is needed, some lines showed trends consistent with a potential improvement in nitrogen use efficiency under EtOH treatment.
For pennycress, we used the spring type line Spring32-10 and evaluated abiotic stress tolerance following EtOH treatment. Assays are underway, and data collection and analysis are ongoing at the time of abstract submission. The results will be presented at the meeting.
This study indicates that EtOH responsiveness is genotype dependent in maize. This finding motivates future genetic dissection, including genome-wide association studies, to identify loci and candidate genes underlying variation in EGAO responses and to deepen mechanistic understanding of ethanol-mediated priming. Ongoing work in pennycress will test whether EGAO can enhance stress resilience in this cover crop and oilseed, which could help stabilize establishment and expand cultivation in marginal environments.
Comment
To browse or post comments, you must log in.Log in