Presentation Information
[2ASPR-17]Analysis and application of the stomatal opening inhibitors to improve drought tolerance
○Shogo Kuwayama1 (1. Nagoya University (Japan))
Keywords:
Stomata,light response,drought tolerance,Compounds
[Purpose]Drought stress is one of the major factors limiting global crop production, and technologies to improve plant drought tolerance are increasingly required. Stomata are small pores on the plant surface that regulate gas exchange and transpiration through opening and closing, thereby playing an essential role in regulating plant water loss. For that reason, regulation of stomatal movement is considered a promising strategy for improving drought tolerance in plants. In this study, we selected some chemical compounds as inhibitors of stomatal opening and evaluated their inhibitory activity in horticultural crops to assess their potential for improving drought tolerance.
[Methode]
We conducted chemical screening to select inhibitors, which suppress light-induced stomatal opening in the epidermis or leaf disc from Commelina benghalensis and Arabidopsis thaliana.
[Results]
Seven candidates were selected based on potency and structural similarity. Among them, we focused on two compounds, P and A, which showed particularly strong inhibitory activity against stomatal opening. Next, we checked the effects of these compounds on stomatal opening in pennycress and tomato. Each compound was applied to leaf surfaces by using a brush. As a result, both compounds significantly suppressed light-induced stomatal opening. Notably, compound P was suggested to inhibit stomatal opening even at nanomolar concentrations.
[Consideration]
These results suggest that the compounds function as effective inhibitors of stomatal opening in horticultural crops, even when applied by simple surface treatment. Therefore, these compounds may have potential as practical chemicals for improving drought tolerance in horticultural plants. Further studies are currently underway to examine the concentration dependence of their effects and their influence on stomatal closure, as well as to evaluate their efficacy under drought stress conditions.
[Conclusion]
We identified chemical compounds that effectively inhibit light-induced stomatal opening in both model plants and horticultural crops. The inhibitory activity was observed even with simple surface application, suggesting their potential as practical agrochemicals for improving drought tolerance. Further evaluation under drought conditions and optimization of application methods will clarify their usefulness for field applications. In this presentation, we will discuss the characterization of these compounds and their potential for field applications.
[Methode]
We conducted chemical screening to select inhibitors, which suppress light-induced stomatal opening in the epidermis or leaf disc from Commelina benghalensis and Arabidopsis thaliana.
[Results]
Seven candidates were selected based on potency and structural similarity. Among them, we focused on two compounds, P and A, which showed particularly strong inhibitory activity against stomatal opening. Next, we checked the effects of these compounds on stomatal opening in pennycress and tomato. Each compound was applied to leaf surfaces by using a brush. As a result, both compounds significantly suppressed light-induced stomatal opening. Notably, compound P was suggested to inhibit stomatal opening even at nanomolar concentrations.
[Consideration]
These results suggest that the compounds function as effective inhibitors of stomatal opening in horticultural crops, even when applied by simple surface treatment. Therefore, these compounds may have potential as practical chemicals for improving drought tolerance in horticultural plants. Further studies are currently underway to examine the concentration dependence of their effects and their influence on stomatal closure, as well as to evaluate their efficacy under drought stress conditions.
[Conclusion]
We identified chemical compounds that effectively inhibit light-induced stomatal opening in both model plants and horticultural crops. The inhibitory activity was observed even with simple surface application, suggesting their potential as practical agrochemicals for improving drought tolerance. Further evaluation under drought conditions and optimization of application methods will clarify their usefulness for field applications. In this presentation, we will discuss the characterization of these compounds and their potential for field applications.
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