Presentation Information
[2ASPR-18]Transcriptome Analysis of Tomato Fruits with CRISPR/Cas9-Mediated Enhanced γ-Aminobutyric Acid (GABA) Accumulation Under Salt Stress.
○Toon Suzuki1 (1. University of Tsukuba (Japan))
Keywords:
Transcriptome,Abiotic Stress,Tomato
[Purpose]
We previously found that a CRISPR/Cas9-engineered high-GABA tomato accumulates even more γ-aminobutyric acid (GABA) under salt stress.
Under salt stress, the cultivar mitigated yield loss compared with the wild type.
In addition, we did not observe decreases in fruit soluble solids (Brix) or in glutamate, a GABA precursor and an umami-related amino acid.
This study aims to identify the mechanisms that support both stress tolerance and fruit quality, and to explore strategies to further improve productivity.
[Method]
We used the commercial cultivar Sicilian Rouge (SR) and a CRISPR/Cas9-derived high-GABA cultivar (SRHG).
Plants were grown in a greenhouse in Japan under four conditions combining genotype and treatment.
Salt stress was applied at EC = 8.0.
Fruits were collected at four ripening stages (IMG, MG, Yel, and Red).
We performed RNA-seq on these samples.
[Results]
Preliminary analyses suggest that GABA modulates the expression of genes related to the MAPK cascade.
In the High GABA cultivar, we also observed patterns consistent with enhancement of alternative GABA-producing routes beyond the major glutamate-dependent pathway.
[Consideration]
These RNA-seq datasets provide important insights into transcriptional regulation linked to the GABA metabolic network and abiotic stress tolerance.
[Conclusion]
GABA may be a key factor enabling both stress tolerance and the production of high-quality fruits.
These findings suggest that metabolic regulation aimed at further increasing GABA could support climate-resilient, high-value agriculture.
We previously found that a CRISPR/Cas9-engineered high-GABA tomato accumulates even more γ-aminobutyric acid (GABA) under salt stress.
Under salt stress, the cultivar mitigated yield loss compared with the wild type.
In addition, we did not observe decreases in fruit soluble solids (Brix) or in glutamate, a GABA precursor and an umami-related amino acid.
This study aims to identify the mechanisms that support both stress tolerance and fruit quality, and to explore strategies to further improve productivity.
[Method]
We used the commercial cultivar Sicilian Rouge (SR) and a CRISPR/Cas9-derived high-GABA cultivar (SRHG).
Plants were grown in a greenhouse in Japan under four conditions combining genotype and treatment.
Salt stress was applied at EC = 8.0.
Fruits were collected at four ripening stages (IMG, MG, Yel, and Red).
We performed RNA-seq on these samples.
[Results]
Preliminary analyses suggest that GABA modulates the expression of genes related to the MAPK cascade.
In the High GABA cultivar, we also observed patterns consistent with enhancement of alternative GABA-producing routes beyond the major glutamate-dependent pathway.
[Consideration]
These RNA-seq datasets provide important insights into transcriptional regulation linked to the GABA metabolic network and abiotic stress tolerance.
[Conclusion]
GABA may be a key factor enabling both stress tolerance and the production of high-quality fruits.
These findings suggest that metabolic regulation aimed at further increasing GABA could support climate-resilient, high-value agriculture.
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