Presentation Information
[2ASPR-19]Multi-omics Analysis to Elucidate Soil Volatile Organic Compounds Dynamics in Soybean Field
○Suzuka Matsuki1 (1. University of Tsukuba (Japan))
Keywords:
Soybean,Soil,VOC,Metabolomics,Multi-omics analysis
Soil is a complex system formed by physiochemical and biological processes that provide essential ecosystem services. In agriculture, soil is crucial for crop cultivation. Within this ecosystem, plantes and soil organisms produce various metabolites into soil. These compounds are not only end products of their genetic information but also participate in feedback regulation for upstream processes. Therefore, understanding soil metabolites is key to clarifying soil functions. However, our knowledge of how soil organisms and plants interact with each other through soil remains limited, especially under natural field conditions.
Among these metabolites, volatile organic compounds (VOCs) are known to be secondary metabolites involved in biotic and abiotic stress response, but reports of soil VOC dynamics remain scarce. In this study, we focused on the relationship between soil VOCs, minerals and microbes in soybean cultivated soil.
We conducted stress applied soybean cultivation trials under stress conditions in both the field and greenhouse to collect bulk and rhizosphere soil samples. We analyzed soil VOCs using HS-SPME-GC/MS method and measured soil mineral concentrations using MP-AES. In addition, we performed 16S rRNA amplicon sequencing to analyze bacterial communities.
Our results revealed that the VOC profiles of the rhizosphere soil were significantly more complex and abundant than those of the bulk soil. In contrast, soil bacterial diversity was lower in the rhizosphere soil. We also found differences in soil mineral concentrations between these two soil types.
These findings provide novel insights into in situ soil VOC composition and highlight the potential for elucidating VOC-mediated biotic interactions in the field. Furthermore, multi-omics analysis revealed the relationships between soil components.
Among these metabolites, volatile organic compounds (VOCs) are known to be secondary metabolites involved in biotic and abiotic stress response, but reports of soil VOC dynamics remain scarce. In this study, we focused on the relationship between soil VOCs, minerals and microbes in soybean cultivated soil.
We conducted stress applied soybean cultivation trials under stress conditions in both the field and greenhouse to collect bulk and rhizosphere soil samples. We analyzed soil VOCs using HS-SPME-GC/MS method and measured soil mineral concentrations using MP-AES. In addition, we performed 16S rRNA amplicon sequencing to analyze bacterial communities.
Our results revealed that the VOC profiles of the rhizosphere soil were significantly more complex and abundant than those of the bulk soil. In contrast, soil bacterial diversity was lower in the rhizosphere soil. We also found differences in soil mineral concentrations between these two soil types.
These findings provide novel insights into in situ soil VOC composition and highlight the potential for elucidating VOC-mediated biotic interactions in the field. Furthermore, multi-omics analysis revealed the relationships between soil components.
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