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[2BRBP-13]Microbial Biorefineries: Converting Agro-Industrial Waste into Bioactive Lipids

○Milan Certik1, Peter Gajdos1, Veronika Hambalko1, Tatiana Klempova1 (1. Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava (Slovakia))
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Keywords:

oleaginous microorganisms,waste conversion,bioactive lipids

Waste management is a significant challenge in the agro-industrial sector. One of the most promising solutions is based on biotechnological techniques applying specific microorganisms for effective utilization of wastes into marketable products. Of them, oleaginous microorganisms are an interesting option to valorize many residual carbon sources to biologically active lipophilic compounds (BALC). Two basic biotechnological processes have been developed for microbial BALC production: submerged and solid-state fermentations. Both fermentation processes provide specific advantages in each industrial application that in turn depends on the bioproduct form, metabolite yield and cost of the production process. The interest has been paid to Zygomycetous oleaginous fungi (Thamnidium sp., Cunninghamella sp., Mucor sp., Umbelopsis sp., Mortierella sp.) and yeasts (Yarrowia lipolytica) that are able to synthesize substantial amounts of BALC from renewable resources.

Filamentous fungi have been effectively used for solid-state fermentation processes, where they transformed waste agroindustrial substrates to fermented bioproducts containing BALC. Depending on the fungal strain and cultivation conditions, a range of agro-based bioproducts have been prepared and successfully employed for making cereal goods and tested as a feed additive for animal diet. Another target is focused on genetically engineered oleaginous yeast Y. lipolytica forming unusual lipid structures. The yeast growing on various wastes (e.g. molasses, glycerol, cooking oil) showed great potential for biosynthesis of unusual and commercially attractive value-added lipid compounds.

Thus, these biotechnological techniques are important contribution to biorefinery field and provide innovative perspectives for bioeconomy by rational optimization of resource usage, minimizing waste, and producing a diverse range of bio-based products.

The work was supported by grants APVV-17-0262, APVV-20-0348, APVV-22-0235 (Slovak Research and Development Agency).

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