[2S06m] 15. Advanced Cell and Cell Culture Engineering for Biopharmaceuticals and Functional Food - Part 4

10:50 AM - 11:10 AM JST(1:50 AM - 2:10 AM UTC)

[2ACCE-04] Metabolomic and Functional Insights into the Mitochondrial Protective Effects of Moringa oleifera Leaf Extracts (MOLE) in Saccharomyces cerevisiae

○Surakrit Suksakul^1,2, Kataleeya Thomrongchote^1,2, Choowong Auesukaree^1,2 (1. Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand (Thailand), 2. Mahidol University-Osaka University Collaborative Research Center for Bioscience and Biotechnology (MU-OU: CRC), Faculty of Science, Mahidol University, Bangkok 10400, Thailand (Thailand))

Keywords:

Mitochondrial dysfunction,Metabolomics,Phytochemicals,Moringa oleifera,Saccharomyces cerevisiae

Mitochondrial dysfunction is a major driver of aging and numerous age-related diseases, whose prevalence continues to increase as populations age. Plant-derived phytochemicals, particularly polyphenols, have emerged as promising candidates for mitigating mitochondrial dysfunction. M. oleifera leaves are a rich source of polyphenols, flavonoids, and other bioactive phytochemicals with reported antioxidant and cytoprotective properties. However, the impact of extraction methods on mitochondrial-targeted bioactivity remains poorly understood. In this study, LC–MS–based untargeted metabolomics was employed to comprehensively characterize the phytochemical profiles of aqueous (AMOLE) and ethanolic (EMOLE) extracts of M. oleifera leaves and to evaluate their protective effects against carbonyl cyanide m-chlorophenyl hydrazone (CCCP)–induced mitochondrial stress in the eukaryotic model organism, S. cerevisiae. Metabolomic profiling revealed distinct solvent-dependent phytochemical signatures. AMOLE was enriched in hydrophilic polyphenols and organic acids, whereas EMOLE contained predominantly less polar phenolic constituents. In functional assays, AMOLE exhibited markedly stronger mitochondrial protective effects, significantly enhancing yeast cell growth, preserving ATP production and mitochondrial membrane potential (MMP), and reducing intracellular reactive oxygen species (ROS) under CCCP-induced stress. These effects were further associated with the modulation of mitochondrial stress-responsive genes. Collectively, our findings reveal that the choice of extraction solvent strongly influences the mitochondrial-protective activity of MOLE and highlight the value of metabolomics-guided strategies for identifying plant-derived interventions against mitochondrial dysfunction.