Presentation Information
[3GET-09-KL]Towards new tools of genome-editing and genome-trimming for microbial synthetic biology
○Qunxin She1 (1. Shandong University (China))
Keywords:
CRISPR-based genome editing,Genome trimming,Microbial chassis cells
[Purpose] Developing new genome editing methods for archaea and bacteria
[Method] CRISPR-based/TnpB-based gene-editing tools and RedET genetic engineering
[Results] Our research on RNA-guided TnpB nucleases has enabled gene-editing at single nucleotide level both in archaea and in bacteria. In addition, we have also developed a new genetic scheme for identification of unknown essential and quasi-essential genes in microbial genomes and replace the original genomic segment using synthetic DNA fragments carrying assembly of essential genes. We also demonstrate that utilizing both Cas9 gene-editing and RedET recombineering further facilitates the genome-trimming process in bacteria.
[Consideration]
[Conclusion] New gene-editing methods have been developed for both archaea and bacteria and they can be tested in other archaea and bacteria.
[Method] CRISPR-based/TnpB-based gene-editing tools and RedET genetic engineering
[Results] Our research on RNA-guided TnpB nucleases has enabled gene-editing at single nucleotide level both in archaea and in bacteria. In addition, we have also developed a new genetic scheme for identification of unknown essential and quasi-essential genes in microbial genomes and replace the original genomic segment using synthetic DNA fragments carrying assembly of essential genes. We also demonstrate that utilizing both Cas9 gene-editing and RedET recombineering further facilitates the genome-trimming process in bacteria.
[Consideration]
[Conclusion] New gene-editing methods have been developed for both archaea and bacteria and they can be tested in other archaea and bacteria.
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