Presentation Information
[3FMBS-07]Profiling cellular dynamics by omics
○Hirofumi Shintaku1 (1. Kyoto University (Japan))
Keywords:
single cell,mechano biology,sequencing,traction force,cell surface tension
The mechanical properties of both cells and their microenvironment are critical determinants of cellular states and functions. We present high-throughput approaches for profiling these mechano-phenotypes at the single-cell level and integrating them with gene expression data.
To characterize cellular mechanical properties, we developed ELASTomics—Electroporation-based Lipid-Assay for Surface Tension and transcriptomics. ELASTomics uniquely harnesses nanopore electroporation, which creates transient pores in lipid bilayers in a cell surface tension–dependent manner. This enables profiling of cell surface tension by delivering DNA-tagged dextran molecules into cells. Using ELASTomics, we uncover mechanical heterogeneity among cells and identify regulatory mechanisms linked to cancer malignancy, cell differentiation, and senescence.
To investigate how cancer cells respond to their mechanical microenvironment, we developed MECH-seq—Micro-Environmental Confinement and Hydrogel-bead cytometry with sequencing. MECH-seq profiles the proliferative behavior of cancer cells under mechanical confinement using hydrogel beads at high throughput. Since cancer cells encounter mechanical stress in both primary and metastatic sites, understanding their responses is crucial. We show that micro-mechanical confinement suppresses the proliferation of breast cancer cells and induces a dormant state.
To characterize cellular mechanical properties, we developed ELASTomics—Electroporation-based Lipid-Assay for Surface Tension and transcriptomics. ELASTomics uniquely harnesses nanopore electroporation, which creates transient pores in lipid bilayers in a cell surface tension–dependent manner. This enables profiling of cell surface tension by delivering DNA-tagged dextran molecules into cells. Using ELASTomics, we uncover mechanical heterogeneity among cells and identify regulatory mechanisms linked to cancer malignancy, cell differentiation, and senescence.
To investigate how cancer cells respond to their mechanical microenvironment, we developed MECH-seq—Micro-Environmental Confinement and Hydrogel-bead cytometry with sequencing. MECH-seq profiles the proliferative behavior of cancer cells under mechanical confinement using hydrogel beads at high throughput. Since cancer cells encounter mechanical stress in both primary and metastatic sites, understanding their responses is crucial. We show that micro-mechanical confinement suppresses the proliferation of breast cancer cells and induces a dormant state.
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