Presentation Information
[3EMT-07]Enhanced biohydrogen and short-chain fatty acid production by immobilized Enterococcus faecalis isolate VT-H1 using food waste as a substrate
Sasithorn Rungjaroenchaiwat1, Thamonwan Woraruthai1, ○Thanyaporn Wongnate1 (1. Vidyasirimedhi Institute of Science and Technology (VISTEC) (Thailand))
Keywords:
Biohydrogen,Dark fermentation,Immobilized cells,Food waste valorization,Enterococcus faecalis
[Purpose] To enhance hydrogen yield, process stability, and cost-effectiveness in biohydrogen production from organic substrates using an immobilized microbial system.
[Method] Enterococcus faecalis VT-H1 was immobilized in calcium alginate beads and evaluated for hydrogen production from glucose, sucrose, and real food waste. The performance of immobilized cells was compared with suspended cells. Key parameters, including alginate concentration and inoculum density, were optimized. Reusability and economic feasibility were also assessed, along with structural analysis using scanning electron microscopy.
[Results] Immobilized cells showed superior performance compared to suspended cells, achieving higher hydrogen yields across all substrates. For example, a yield of 1.712 mol H2/mol glucose was obtained compared to 1.362 mol H2/mol glucose from free cells. Optimal conditions were 2% alginate and OD600 = 1.0, resulting in enhanced hydrogen production rates and reduced lag phase. The immobilized system exhibited strong tolerance to substrate inhibition and maintained efficient hydrogen production even with undiluted food waste. Reusability was demonstrated over three consecutive cycles with moderate performance decline. Economic analysis indicated reduced operating costs due to biocatalyst reuse. SEM analysis revealed increased porosity and cell proliferation within the alginate matrix.
[Consideration] Immobilization improves cell retention, enhances microbial activity, and provides protection against inhibitory conditions, contributing to improved system robustness and operational stability.
[Conclusion] Alginate-immobilized Enterococcus faecalis VT-H1 significantly improves hydrogen production efficiency, stability, and cost-effectiveness. This approach offers strong potential for scalable biohydrogen production from organic waste within a circular bioeconomy framework.
[Method] Enterococcus faecalis VT-H1 was immobilized in calcium alginate beads and evaluated for hydrogen production from glucose, sucrose, and real food waste. The performance of immobilized cells was compared with suspended cells. Key parameters, including alginate concentration and inoculum density, were optimized. Reusability and economic feasibility were also assessed, along with structural analysis using scanning electron microscopy.
[Results] Immobilized cells showed superior performance compared to suspended cells, achieving higher hydrogen yields across all substrates. For example, a yield of 1.712 mol H2/mol glucose was obtained compared to 1.362 mol H2/mol glucose from free cells. Optimal conditions were 2% alginate and OD600 = 1.0, resulting in enhanced hydrogen production rates and reduced lag phase. The immobilized system exhibited strong tolerance to substrate inhibition and maintained efficient hydrogen production even with undiluted food waste. Reusability was demonstrated over three consecutive cycles with moderate performance decline. Economic analysis indicated reduced operating costs due to biocatalyst reuse. SEM analysis revealed increased porosity and cell proliferation within the alginate matrix.
[Consideration] Immobilization improves cell retention, enhances microbial activity, and provides protection against inhibitory conditions, contributing to improved system robustness and operational stability.
[Conclusion] Alginate-immobilized Enterococcus faecalis VT-H1 significantly improves hydrogen production efficiency, stability, and cost-effectiveness. This approach offers strong potential for scalable biohydrogen production from organic waste within a circular bioeconomy framework.
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