Presentation Information
[3CTBP-10]Collagen-based Viscous Matrices: Advancing the Frontiers of Tissue Engineering
○Michiya Matsusaki1 (1. The University of Osaka (Japan))
Keywords:
Tissue engineering,Viscous matrix
In the body, tissues and organs are composed of cells and extracellular matrices that support cell adhesion, growth, and differentiation. Tissue engineering generally involves two main approaches: top-down and bottom-up. In the top-down approach, porous scaffolds are first fabricated, and cells are subsequently seeded into these structures. In contrast, the bottom-up approach begins with the preparation of cell sheets or cell spheroids, which are then assembled to construct three-dimensional (3D) tissue-like structures. Both approaches have their advantages and limitations. However, fabricating complex tissues that incorporate aligned cell fibers, layered cell sheets, and cell spheroids at defined positions within three-dimensional (3D) structures remains a major challenge in this field.
We recently developed a novel in situ tissue engineering approach using collagen-based viscous matrices (VMs). When collagen is simply mixed with anionic polyelectrolytes, VMs rapidly form within seconds through the entanglement of collagen molecules. Because these matrices retain the cell-adhesive sequences of collagen, they support cell adhesion, enabling the formation of tissue-like constructs by simply mixing VMs with cells. We have already demonstrated the fabrication of hepatocyte tissues and cancer–stromal tissues using the VM method. Furthermore, we recently established a fundamental strategy for in situ fabrication of dimension-controlled, cell-laden constructs, including fibers (1D), capsules (0D), sheets (2D), and tubes (1D). These dimensionally controlled building units can serve as modular components for assembling complex three-dimensional tissue-like structures.
We recently developed a novel in situ tissue engineering approach using collagen-based viscous matrices (VMs). When collagen is simply mixed with anionic polyelectrolytes, VMs rapidly form within seconds through the entanglement of collagen molecules. Because these matrices retain the cell-adhesive sequences of collagen, they support cell adhesion, enabling the formation of tissue-like constructs by simply mixing VMs with cells. We have already demonstrated the fabrication of hepatocyte tissues and cancer–stromal tissues using the VM method. Furthermore, we recently established a fundamental strategy for in situ fabrication of dimension-controlled, cell-laden constructs, including fibers (1D), capsules (0D), sheets (2D), and tubes (1D). These dimensionally controlled building units can serve as modular components for assembling complex three-dimensional tissue-like structures.
Comment
To browse or post comments, you must log in.Log in