[3S06m] 16. Cell and Tissue Engineering for Biomedical Process Innovation - Part 1

10:00 AM - 10:40 AM JST(1:00 AM - 1:40 AM UTC)

[3CTBP-02-KL] Tissue engineering for hair regenerative medicine

○Junji Fukuda^1,2,3 (1. Yokohama National University (Japan), 2. Kanagawa Institute of Industrial Science and Technology (Japan), 3. TrichoSeeds (Japan))

Keywords:

hair regenerative medicine,dermal papilla cells,hair follicle organoid

Hair regenerative medicine generally categorizes into three major approaches. The first approach (HR01) is to activate miniaturized hair follicles by transplanting dermal papilla cells, specialized mesenchymal cells that regulate hair follicle development and cycle. The second approach (HR02) is to generate de novo hair follicles by transplanting engineered tissues composed of dermal papilla cells and hair follicle stem cells. The third approach (HR03) is to transplant hair follicles generated in vitro in organoid culture. For any of these three approaches, methods to expand the number of one (dermal papilla cells for HR01) or two (dermal papilla and epithelial stem cells for HR02, 03) cell types are required to prepare a large number of tissue grafts for hair regeneration. We found a novel culture method capable of growing dermal papilla cells while maintaining their hair growth activity, with which we are currently working toward approval for clinical trial of HR01. The mechanisms responsible for the maintenance of the activity may be involved in low oxygen partial pressure, extracellular matrix accumulation, and autocrine extracellular vesicles. In HR02, hair follicle germ-like tissue grafts were prepared and showed highly efficient hair follicle generation upon transplantation into the skin of mice (1,2). In this approach, control over oxygen tension in culture environment was crucial. Currently, we are working with the cells from androgenic alopecia patients, instead of cells from mouse origin. To investigate the feasibility of HR03, the hair follicles were generated in vitro in the organoid culture and transplanted them into mouse skin as seen in hair transplantation (3,4). In my talk, the importance of control of oxygen tension in these approaches and the challenges will be discussed.
1) E. Sugiyama, A. Nanmo, N. Xiaolei, SY. Chang, M. Hashimoto, A. Suzuki, T. Kageyama, J. Fukuda, Large-scale preparation of hair follicle germs using a microfluidic device, ACS Biomaterials Science & Engineering, 10, 2, 998–1005 (2024)
2) A. Nanmo, L. Yan, T. Asaba, L. Wan, T. Kageyama, and J. Fukuda, Bioprinting of hair follicle germs for hair regenerative medicine, Acta Biomaterialia, 165, 50-59 (2023)
3) T. Kageyama, R. Anakama, S. Hamano, S. Tu, Y. Migita, T. Asaba, A. Nanmo, K. Ishikawa, L. Yan, J. Seo, J. Fukuda, Hair follicle organoids using human iPSC-derived ectodermal precursor cells for hair regenerative medicine, ACS Biomaterials Science & Engineering, 12, 3, 1704–1710 (2026)
4) T. Kageyama, A. Shimizu, R. Anakama, R. Nakajima, K. Suzuki, Y. Okubo, J. Fukuda, Reprogramming of three-dimensional microenvironments for in vitro hair follicle induction, Science Advances, 8(42), eadd4603 (2022)