Presentation Information
[3CTBP-05-KL]Preparation of the collagen/polysaccharide crosslinked glycerol-based gel and application of osteogenesis
○Yoshiro Tahara1, Satoshi Komasa2 (1. Doshisha University (Japan), 2. Osaka Dental University (Japan))
Keywords:
gel,collagen,glycerol,polysaccaride
In tissue engineering, the scaffold for three-dimensional cell culturing is important, and various type polymer crosslinked hydrogels have been developed. However, it is not still enough to meet unmet needs in clinical because it is difficult to control biological and rheological properties by using cost effective modification. In this study, glycerol was used as a continuous liquid media of gels and kept as gel state by crosslinking of collagen and polysaccharides.
The collagen and pullulan crosslinked glycerol gels were prepared and investigated its basic properties [1]. Briefly, collagen, pullulan and glycerol were dissolved in acidic aqueous solution, and pH was adjusted by NaOH. After freeze drying of this mixture, the collagen and pullulan crosslinked glycerol gel was prepared. Collagen hydrogel (water-based gel) was used as a control of traditional and representative scaffold. The significant disadvantage of the collagen hydrogel is its weakness so that it is easy to be dissolved in water and it is not possible to form desired macroscopic structure. On the other hand, the glycol-based gel is not dissolved in water immediately. Importantly, we can insert the glycol-based gel in syringe and transform to desired shape. This injectable and suitable rheological property is very useful for the scaffold of tissue engineering. From the rheological characterization, storage modulus was higher than loss modulus suggesting that glycerol was entrapped in collagen and pullulan polymer network and formed the gel state. The storage modulus of the collagen hydrogel was also higher than loss modulus. However, both values of the glycerol-based gel were over 100-fold higher than that of the collagen hydrogel. In addition, both storage and loss modulus of the glycerol-based gels were changed depending on the frequency, suggesting that the glycerol-based gel is possible to change its shape by exogenous mechanical stress. For the biological application, in the results of culturing mesenchymal stem cells, osteogenesis-related markers were upregulated, and calcium was deposited under osteogenic differentiation condition. The in vivo experiment of repairing the rat cranial defects showed that the glycerol-based gels enhanced bone repair more than the collagen hydrogels.
In conclusion, the collagen and polysaccharide crosslinked glycerol gels showed the beneficial mechanical properties, and it can be useful compared with the traditional collagen hydrogels for tissue engineering such as bone repairing.
[1] X. Wang, S. Komasa, Y. Tahara, S. Inui, M. Matsumoto, K. Maekawa, Novel injectable collagen/glycerol/pullulan gel promotes osteogenic differentiation of mesenchymal stem cells and the repair of rat cranial defects, Gels 10, 775 (2024).
The collagen and pullulan crosslinked glycerol gels were prepared and investigated its basic properties [1]. Briefly, collagen, pullulan and glycerol were dissolved in acidic aqueous solution, and pH was adjusted by NaOH. After freeze drying of this mixture, the collagen and pullulan crosslinked glycerol gel was prepared. Collagen hydrogel (water-based gel) was used as a control of traditional and representative scaffold. The significant disadvantage of the collagen hydrogel is its weakness so that it is easy to be dissolved in water and it is not possible to form desired macroscopic structure. On the other hand, the glycol-based gel is not dissolved in water immediately. Importantly, we can insert the glycol-based gel in syringe and transform to desired shape. This injectable and suitable rheological property is very useful for the scaffold of tissue engineering. From the rheological characterization, storage modulus was higher than loss modulus suggesting that glycerol was entrapped in collagen and pullulan polymer network and formed the gel state. The storage modulus of the collagen hydrogel was also higher than loss modulus. However, both values of the glycerol-based gel were over 100-fold higher than that of the collagen hydrogel. In addition, both storage and loss modulus of the glycerol-based gels were changed depending on the frequency, suggesting that the glycerol-based gel is possible to change its shape by exogenous mechanical stress. For the biological application, in the results of culturing mesenchymal stem cells, osteogenesis-related markers were upregulated, and calcium was deposited under osteogenic differentiation condition. The in vivo experiment of repairing the rat cranial defects showed that the glycerol-based gels enhanced bone repair more than the collagen hydrogels.
In conclusion, the collagen and polysaccharide crosslinked glycerol gels showed the beneficial mechanical properties, and it can be useful compared with the traditional collagen hydrogels for tissue engineering such as bone repairing.
[1] X. Wang, S. Komasa, Y. Tahara, S. Inui, M. Matsumoto, K. Maekawa, Novel injectable collagen/glycerol/pullulan gel promotes osteogenic differentiation of mesenchymal stem cells and the repair of rat cranial defects, Gels 10, 775 (2024).
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