Presentation Information
[4FMBS-11]Biofunctional Eruca sativa-Derived Gold Nanoparticles Formulations for Wound Healing and Inflammation Management
○Younis Baqi1, Abdullah M. Hazbar2, Abdulkadir Mohammed Noori Jassim2, Mustafa Taha Mohammed2 (1. Sultan Qaboos University (Oman), 2. Mustansiriyah University (Iraq))
Keywords:
Eruca sativa,Gold nanoparticles (AuNPs),Toxicity,Oxidative stress,Wound healing
[Purpose]
Gold nanoparticles (AuNPs) hold promise for the therapy of cancer and inflammatory conditions. This study describes the preparation and evaluation of novel creams obtained from AuNPs prepared with E. sativa for capping (“green nanoparticles”, termed Es-AuNPs) and E. sativa leaf powder (Es), a plant known to contain anti-inflammatory constituents. [Method]
Es-AuNPs, E. sativa extract, powder, and the topical formulations (Es-AuNPs and Es) were investigated in vitro and in vivo. GC-MS analysis of the plant extract was performed to identify bioactive phytochemicals that may contribute to its bioactivities. Acute toxicity studies were carried out in Wistar rats. The total oxidant status (TOS) and total antioxidant capacity (TAC) were determined for E. sativa extract and Es-AuNPs. The newly formulated creams were also tested in wound-healing assays in mice to assess their anti-inflammatory activity versus paraffin (negative control) and Fucidin. [Results]
GC-MS analysis revealed the presence of bioactive phytochemicals in the plant extract. Acute toxicity studies showed no mortality in Wistar rats; however, a dose-dependent hepatotoxicity was observed for Es-AuNPs, while E. sativa extract was not hepatotoxic. Determination of TOS for E. sativa extract and low-dose Es-AuNPs showed a reduced oxidant parameter, whereas this parameter was elevated at a high dose of Es-AuNPs, indicating a dual antioxidant/pro-oxidant role. Measurement of TAC revealed a non-significant pattern. In wound-healing assays, applying Es-AuNPs and E. sativa powder creams (1% w/w) promoted wound closure, injured tissue regeneration, decreased inflammation, and facilitated epithelial repair, with Es-AuNPs showing stronger effects than E. sativa. [Consideration]
The presence of bioactive phytochemicals in the plant extract likely contributes to its biological activities. The results indicate that Es-AuNPs exhibit promising therapeutic effects, while their dose-dependent hepatotoxicity suggests that careful dose optimization is necessary for further development. [Conclusion]
These findings suggest promising therapeutic effects of Es-AuNPs- and E. sativa powder-containing creams in skin inflammation management
Gold nanoparticles (AuNPs) hold promise for the therapy of cancer and inflammatory conditions. This study describes the preparation and evaluation of novel creams obtained from AuNPs prepared with E. sativa for capping (“green nanoparticles”, termed Es-AuNPs) and E. sativa leaf powder (Es), a plant known to contain anti-inflammatory constituents. [Method]
Es-AuNPs, E. sativa extract, powder, and the topical formulations (Es-AuNPs and Es) were investigated in vitro and in vivo. GC-MS analysis of the plant extract was performed to identify bioactive phytochemicals that may contribute to its bioactivities. Acute toxicity studies were carried out in Wistar rats. The total oxidant status (TOS) and total antioxidant capacity (TAC) were determined for E. sativa extract and Es-AuNPs. The newly formulated creams were also tested in wound-healing assays in mice to assess their anti-inflammatory activity versus paraffin (negative control) and Fucidin. [Results]
GC-MS analysis revealed the presence of bioactive phytochemicals in the plant extract. Acute toxicity studies showed no mortality in Wistar rats; however, a dose-dependent hepatotoxicity was observed for Es-AuNPs, while E. sativa extract was not hepatotoxic. Determination of TOS for E. sativa extract and low-dose Es-AuNPs showed a reduced oxidant parameter, whereas this parameter was elevated at a high dose of Es-AuNPs, indicating a dual antioxidant/pro-oxidant role. Measurement of TAC revealed a non-significant pattern. In wound-healing assays, applying Es-AuNPs and E. sativa powder creams (1% w/w) promoted wound closure, injured tissue regeneration, decreased inflammation, and facilitated epithelial repair, with Es-AuNPs showing stronger effects than E. sativa. [Consideration]
The presence of bioactive phytochemicals in the plant extract likely contributes to its biological activities. The results indicate that Es-AuNPs exhibit promising therapeutic effects, while their dose-dependent hepatotoxicity suggests that careful dose optimization is necessary for further development. [Conclusion]
These findings suggest promising therapeutic effects of Es-AuNPs- and E. sativa powder-containing creams in skin inflammation management
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