Presentation Information
[4FMBS-01-KL]Enzymatic Intracellular Self-Assembly
○Bing Xu1 (1. Brandeis University (USA))
Keywords:
Enzyme,Self-assembly,Cell,biomedicine,subcellular
[Purpose]
Self-assembly is a fundamental mechanism of intracellular organization, where proteins dynamically assemble and disassemble under precise enzymatic control. Inspired by this biomimetic principle, this work aims to develop and apply enzyme-instructed self-assembly (EISA) as a strategy to generate intracellular supramolecular nanostructures that can modulate cellular behavior and advance therapeutic development.
[Method]
EISA couples enzyme-mediated molecular transformations with self-assembly processes. In this approach, small-molecule precursors are locally converted by specific enzymes into self-assembling peptides, leading to the in situ formation of supramolecular nanostructures inside cells. These assemblies are non-diffusive and can be generated with subcellular precision, enabling targeted formation within specific organelles.
[Results]
EISA enables the intracellular formation of peptide assemblies that emulate natural regulatory pathways. This strategy supports the development of next-generation biomedicines, particularly in anticancer nanomedicine. Representative applications demonstrate that enzymatically generated peptide assemblies can selectively engage multiple subcellular targets, including the plasma membrane, mitochondria, endoplasmic reticulum, Golgi apparatus, and nucleus, for therapeutic purposes.
[Consideration]
As a cell-compatible and highly adaptable strategy, EISA offers conceptual simplicity and distinctive advantages, including its ability to create intracellular peptide architectures with spatial precision. By mimicking cellular regulatory mechanisms, this approach provides a powerful platform for directing cell behavior while maintaining compatibility with complex intracellular environments.
[Conclusion]
Intracellular self-assembly directed by enzymatic processes represents a versatile and innovative platform for therapeutic intervention. By leveraging enzyme-instructed self-assembly, it is possible to generate functional peptide nanostructures within cells, expand organelle-targeted strategies, and advance the landscape of anticancer and translational nanomedicine.
Self-assembly is a fundamental mechanism of intracellular organization, where proteins dynamically assemble and disassemble under precise enzymatic control. Inspired by this biomimetic principle, this work aims to develop and apply enzyme-instructed self-assembly (EISA) as a strategy to generate intracellular supramolecular nanostructures that can modulate cellular behavior and advance therapeutic development.
[Method]
EISA couples enzyme-mediated molecular transformations with self-assembly processes. In this approach, small-molecule precursors are locally converted by specific enzymes into self-assembling peptides, leading to the in situ formation of supramolecular nanostructures inside cells. These assemblies are non-diffusive and can be generated with subcellular precision, enabling targeted formation within specific organelles.
[Results]
EISA enables the intracellular formation of peptide assemblies that emulate natural regulatory pathways. This strategy supports the development of next-generation biomedicines, particularly in anticancer nanomedicine. Representative applications demonstrate that enzymatically generated peptide assemblies can selectively engage multiple subcellular targets, including the plasma membrane, mitochondria, endoplasmic reticulum, Golgi apparatus, and nucleus, for therapeutic purposes.
[Consideration]
As a cell-compatible and highly adaptable strategy, EISA offers conceptual simplicity and distinctive advantages, including its ability to create intracellular peptide architectures with spatial precision. By mimicking cellular regulatory mechanisms, this approach provides a powerful platform for directing cell behavior while maintaining compatibility with complex intracellular environments.
[Conclusion]
Intracellular self-assembly directed by enzymatic processes represents a versatile and innovative platform for therapeutic intervention. By leveraging enzyme-instructed self-assembly, it is possible to generate functional peptide nanostructures within cells, expand organelle-targeted strategies, and advance the landscape of anticancer and translational nanomedicine.
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