[4S05Am] 21. Open Topics in Biotechnology - Part 2

10:05 AM - 10:20 AM JST(1:05 AM - 1:20 AM UTC)

[4Open-01] Electrochemical Mapping of Catecholamine-Copper Redox Cycling Induced DNA Damage Using a MXene-HOF Hybrid Platform.

○Niyas Kannankuzhiyan¹ (1. Indian Institute of Technology Palakkad (India))

Keywords:

Catecholamine,DNA damage,Copper ion,Antioxidants

Oxidative DNA damage induced by reactive oxygen species (ROS) plays a critical role in mutagenesis and the progression of neurodegenerative disorders, with guanine being the most oxidation-susceptible nucleobase forming 8-oxoguanine (8-oxoG) lesions. Catecholamine neurotransmitters, particularly in the presence of transition metals such as Cu²⁺ can undergo redox cycling to generate ROS, thereby accelerating oxidative DNA damage. In this work, we report a novel electrochemical DNA damage probe based on a poly-L-lysine electropolymerized Nb₂CT_x MXene-hydrogen-bonded organic framework nanocomposite modified carbon yarn electrode (pLy-HOF-Nb₂CT_x /CY)for monitoring catecholamine-mediated oxidative guanine damage and evaluating antioxidant protection. The nanocomposite electrode exhibited enhanced electron transfer kinetics and increased electroactive surface area, enabling sensitive detection of 8-oxoG generated at the electrode interface. The voltammetric experiments was demonstrated to characterize the catecholamines (dopamine, epinephrine, norepinephrine, and L-DOPA) and copper ion (Cu²⁺)-mediated guanine oxidation, with further, confirming synergistic metal-mediated oxidative damage. The 8-oxoG peak currents increased markedly in catecholamine-Cu²⁺ systems, indicating enhanced ROS-driven DNA oxidation. The platform was further employed to investigate the protective effects of antioxidants, including ascorbic acid, quercetin, curcumin, resveratrol, glutathione, EGCG, N-acetyl cysteine, and α-lipoic acid, which substantially suppressed the 8-oxoG signal, with several compounds exhibiting >90% inhibition of oxidative damage. These findings demonstrate that the developed MXene-HOF-polylysine electrochemical probe provides a robust and sensitive approach for studying metal-mediated oxidative DNA damage and evaluating antioxidant efficacy. The proposed strategy offers valuable insight into catecholamine-induced genotoxicity and holds promise for screening protective agent against oxidative stress-related diseases.