Presentation Information
[4DSP-01-KL]Challenges in Isolation and Purification Technologies for Extracellular Vesicle Research
○Naoomi Tominaga1 (1. Yamaguchi University (Japan))
Keywords:
Extracellular vesicles,Exosomes,Purification
[Purpose]
Extracellular vesicles (EVs) have attracted growing interest as a basis for next-generation diagnostics and therapeutics. However, owing to their inherent heterogeneity, developing isolation and purification methods that recover target fractions with high purity and reproducibility remains a major bottleneck. This presentation summarizes the current status and challenges of EV isolation technologies in light of MISEV2023, the latest guidelines published by the International Society for Extracellular Vesicles (ISEV).
[Results]
We present comparative data from our laboratory obtained using multiple isolation principles and describe how different recovery methods affect outcomes. The choice of isolation method has been reported to alter the characteristics of recovered particles and their molecular profiles, directly affecting the interpretation of downstream omics analyses and biological activity assessments. We demonstrate practical examples in which different purification processes yield divergent experimental results from the same starting material. Furthermore, we discuss how changes in cell culture conditions or physiological state influence the quantity and quality of EV secretion, and examine the current limitations in the power of existing isolation technologies.
[Conclusion]
For the translational application of EVs, it is essential to establish purification processes capable of distinguishing biologically meaningful changes from technical noise. Technologies that go beyond ensuring yield and can selectively isolate target subsets reflecting specific cellular states are needed. In summary, robust isolation technology is a cornerstone of quality control (QC) in EV manufacturing, and overcoming its associated challenges will be pivotal for advancing future bioprocess development.
Extracellular vesicles (EVs) have attracted growing interest as a basis for next-generation diagnostics and therapeutics. However, owing to their inherent heterogeneity, developing isolation and purification methods that recover target fractions with high purity and reproducibility remains a major bottleneck. This presentation summarizes the current status and challenges of EV isolation technologies in light of MISEV2023, the latest guidelines published by the International Society for Extracellular Vesicles (ISEV).
[Results]
We present comparative data from our laboratory obtained using multiple isolation principles and describe how different recovery methods affect outcomes. The choice of isolation method has been reported to alter the characteristics of recovered particles and their molecular profiles, directly affecting the interpretation of downstream omics analyses and biological activity assessments. We demonstrate practical examples in which different purification processes yield divergent experimental results from the same starting material. Furthermore, we discuss how changes in cell culture conditions or physiological state influence the quantity and quality of EV secretion, and examine the current limitations in the power of existing isolation technologies.
[Conclusion]
For the translational application of EVs, it is essential to establish purification processes capable of distinguishing biologically meaningful changes from technical noise. Technologies that go beyond ensuring yield and can selectively isolate target subsets reflecting specific cellular states are needed. In summary, robust isolation technology is a cornerstone of quality control (QC) in EV manufacturing, and overcoming its associated challenges will be pivotal for advancing future bioprocess development.
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