Presentation Information
[P01-059]Metabolic Engineering of Glucose/Glycerol Utilization for Improved Acetoin Production in Bacillus subtilis
Po-Hsun Chou1, ○Po-Ting Chen1 (1. Southern Taiwan University of Science and Technology (Taiwan))
Keywords:
Acetoin,Bacillus subtilis,Glycerol
[Purpose]
Acetoin is a platform chemical with broad industrial applications, and Bacillus subtilis (GRAS) is a suitable host for sustainable acetoin biosynthesis. This study aimed to improve acetoin production by integrating global regulatory tuning with enhanced carbon uptake.
[Method]
A metabolic engineering strategy was evaluated by combining a global regulatory intervention with either a glucose transport module (GlcU) or a glycerol uptake operon (GlpFK). Engineered strains were first assessed in shake-flask cultures using glycerol as the sole carbon source, followed by batch cultivation in a 5-L bioreactor without pH control.
[Results]
Among the engineered strains, ACN16 showed the best performance, achieving a 2.91-fold increase in acetoin production in shake flasks with glycerol as the sole carbon source. In a 5-L bioreactor, batch fermentation without pH control resulted in a further 1.78-fold increase in acetoin production after 72 h compared with shake-flask cultivation.
[Consideration]
The results indicate that improving glycerol assimilation coupled with global regulatory tuning can increase carbon flux toward acetoin. The observed enhancement under non-pH-controlled bioreactor conditions suggests the approach may be robust across cultivation formats, although additional optimization may be required for process control and reproducibility.
[Conclusion]
These findings suggest that combining global regulatory tuning with enhanced glycerol uptake is a viable strategy to improve acetoin production in B. subtilis and support glycerol as a potential low-cost substrate for acetoin fermentation.
Acetoin is a platform chemical with broad industrial applications, and Bacillus subtilis (GRAS) is a suitable host for sustainable acetoin biosynthesis. This study aimed to improve acetoin production by integrating global regulatory tuning with enhanced carbon uptake.
[Method]
A metabolic engineering strategy was evaluated by combining a global regulatory intervention with either a glucose transport module (GlcU) or a glycerol uptake operon (GlpFK). Engineered strains were first assessed in shake-flask cultures using glycerol as the sole carbon source, followed by batch cultivation in a 5-L bioreactor without pH control.
[Results]
Among the engineered strains, ACN16 showed the best performance, achieving a 2.91-fold increase in acetoin production in shake flasks with glycerol as the sole carbon source. In a 5-L bioreactor, batch fermentation without pH control resulted in a further 1.78-fold increase in acetoin production after 72 h compared with shake-flask cultivation.
[Consideration]
The results indicate that improving glycerol assimilation coupled with global regulatory tuning can increase carbon flux toward acetoin. The observed enhancement under non-pH-controlled bioreactor conditions suggests the approach may be robust across cultivation formats, although additional optimization may be required for process control and reproducibility.
[Conclusion]
These findings suggest that combining global regulatory tuning with enhanced glycerol uptake is a viable strategy to improve acetoin production in B. subtilis and support glycerol as a potential low-cost substrate for acetoin fermentation.
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