[P001] Poster

12:10 PM - 1:00 PM JST(3:10 AM - 4:00 AM UTC)

[P01-085] Investigation of overlapping methionine biosynthetic pathways in Streptomyces fradiae NBRC 12773

○Souma Yoshida¹, Chitose Maruyama¹, Yoshimitsu Hamano¹, Fumihito Hasebe¹ (1. Fukui Prefectural University (Japan))

Keywords:

methionine biosynthesis,Streptomyces,overlapping pathway

Methionine is a sulfur-containing proteinogenic amino acid. It also functions as the immediate precursor to S-adenosyl-l-methionine, a universal methyl donor used in primary and secondary metabolism. Recently, our group reported the identification of a novel gene (metM) responsible for methionine biosynthesis in Streptomyces albulus NBRC 14147 and demonstrated that the gene product, MetM, is a novel homocysteine synthase^[1]. The metM homologs are also distributed in other Streptomyces strains^[1].Based on genome analysis, Streptomyces fradiae NBRC 12773, a neomycin-producing actinomycete, also possesses a metM homolog (SfmetM), indicating that this strain uses SfmetM for methionine biosynthesis. Interestingly, in addition to SfmetM, this strain possesses canonical methionine biosynthetic gene homologs (SfmetA and SfmetC), suggesting overlapping methionine biosynthetic pathways. Although the biological significance of the overlap is unclear, it may be involved in the growth benefits and the production of natural products. Thus, in this study, we address the question of overlapping methionine biosynthetic pathways in S. fradiae NBRC 12773.

Reference
[1] F. Hasebe et al. Appl. Environ. Microbiol. 90, e01247-24 (2024).