[P001] Poster

12:10 PM - 1:00 PM JST(3:10 AM - 4:00 AM UTC)

[P01-103] Heterologous Production of Medium-Chain-Length Polyhydroxyalkanoates in Engineered Streptomyces Strains

○Pamella Apriliana¹, Prihardi Kahar¹, Chiaki Ogino¹ (1. Kobe university (Japan))

Keywords:

Streptomyces,polyhydroxyalkanoates,copolymer

[Purpose]
The growing demand for sustainable and biodegradable materials has driven interest in microbial production of polyhydroxyalkanoates (PHAs). Streptomyces species, known for their metabolic versatility and ability to utilize diverse carbon sources, represent promising yet underexplored hosts for biotechnological applications. This study aimed to evaluate the feasibility of engineering Streptomyces strains for the biosynthesis of medium-chain-length PHAs (mcl-PHAs) through heterologous gene expression.

[Method]
A synthetic PHA biosynthesis gene cluster (ABCGC cassette), consisting of phbA, phbB, phaC1, phaG, and phaC2, was introduced into three Streptomyces strains: Streptomyces albus, Streptomyces coelicolor, and Streptomyces maritimus. The engineered strains were cultivated under controlled conditions using sodium octanoate as the primary carbon source to induce PHA production. Polymer synthesis was subsequently evaluated to determine the capability of these modified strains to produce biodegradable polyesters.
[Results]
The engineered Streptomyces strains successfully synthesized medium-chain-length PHAs, specifically the copolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) [P(3HB-co-3HHx-co-3HO)]. This result demonstrates that Streptomyces can function as a viable host for heterologous PHA production despite the absence of previously established genetic engineering systems for this purpose. The produced copolymer is of particular interest due to its favorable material properties and potential applications in the biomedical field.

[Conclusion]
This study provides the first evidence supporting the feasibility of using genetically engineered Streptomyces for mcl-PHA production. The findings highlight the organism’s potential as a platform for sustainable biopolymer synthesis and contribute to the advancement of environmentally friendly material production systems. This work lays the foundation for further optimization and development of Streptomyces-based bioprocesses aimed at reducing environmental impact and promoting the use of renewable resources in industrial biotechnology.