Presentation Information
[P01-149]Postbiotics Derived from Leuconostoc mesenteroides as Multifunctional Bioactive Agents for Protection against Blue Light–Induced Skin Photoaging
○Shih Chun Yang1 (1. Department of Microbiology, Soochow University, Taiwan (Taiwan))
Keywords:
Leuconostoc mesenteroides,blue light,photoaging,postbiotics
[Purpose]
High-energy visible light (HEV), especially blue light, has emerged as a critical environmental factor contributing to skin photoaging through the induction of oxidative stress, inflammation, and melanogenesis. This study aimed to investigate the protective effects of postbiotics derived from Leuconostoc mesenteroides against blue light–induced skin damage and to evaluate their potential as multifunctional bioactive ingredients for skincare applications.
[Method]
A heterofermentative strain of L. mesenteroides was screened and cultured, and its fermented cell lysates and postbiotic preparations were obtained. Blue light–induced oxidative stress models were established using HaCaT keratinocytes, while melanogenesis models were conducted in B16-F10 melanoma cells. Intracellular ROS levels were measured, and the protein expression of inflammatory markers (iNOS, COX-2) and melanogenesis-related proteins (MITF, tyrosinase, TRP-1, TRP-2) were analyzed. Cell migration and wound healing activity were also evaluated using in vitro scratch assays.
[Results]
L. mesenteroides–derived cell lysates and postbiotics significantly suppressed intracellular ROS accumulation in blue light–irradiated HaCaT cells and markedly reduced iNOS and COX-2 protein expression. In B16-F10 cells, these preparations significantly downregulated the expression of MITF, tyrosinase, TRP-1, and TRP-2 under blue light exposure. In addition, the postbiotics promoted cell migration and enhanced wound healing activity.
[Consideration]
The observed antioxidant, anti-inflammatory, and anti-melanogenic effects suggest that L. mesenteroides postbiotics modulate multiple molecular pathways associated with blue light–induced skin damage. These multifunctional activities indicate a synergistic mechanism contributing to overall skin protection and regeneration.
[Conclusion]
Postbiotics derived from L. mesenteroides exhibit potent protective effects against blue light–induced oxidative stress, inflammation, and melanogenesis, while also promoting wound healing. These findings support their potential development as novel multifunctional bioactive materials for functional skincare and medical aesthetic applications.
High-energy visible light (HEV), especially blue light, has emerged as a critical environmental factor contributing to skin photoaging through the induction of oxidative stress, inflammation, and melanogenesis. This study aimed to investigate the protective effects of postbiotics derived from Leuconostoc mesenteroides against blue light–induced skin damage and to evaluate their potential as multifunctional bioactive ingredients for skincare applications.
[Method]
A heterofermentative strain of L. mesenteroides was screened and cultured, and its fermented cell lysates and postbiotic preparations were obtained. Blue light–induced oxidative stress models were established using HaCaT keratinocytes, while melanogenesis models were conducted in B16-F10 melanoma cells. Intracellular ROS levels were measured, and the protein expression of inflammatory markers (iNOS, COX-2) and melanogenesis-related proteins (MITF, tyrosinase, TRP-1, TRP-2) were analyzed. Cell migration and wound healing activity were also evaluated using in vitro scratch assays.
[Results]
L. mesenteroides–derived cell lysates and postbiotics significantly suppressed intracellular ROS accumulation in blue light–irradiated HaCaT cells and markedly reduced iNOS and COX-2 protein expression. In B16-F10 cells, these preparations significantly downregulated the expression of MITF, tyrosinase, TRP-1, and TRP-2 under blue light exposure. In addition, the postbiotics promoted cell migration and enhanced wound healing activity.
[Consideration]
The observed antioxidant, anti-inflammatory, and anti-melanogenic effects suggest that L. mesenteroides postbiotics modulate multiple molecular pathways associated with blue light–induced skin damage. These multifunctional activities indicate a synergistic mechanism contributing to overall skin protection and regeneration.
[Conclusion]
Postbiotics derived from L. mesenteroides exhibit potent protective effects against blue light–induced oxidative stress, inflammation, and melanogenesis, while also promoting wound healing. These findings support their potential development as novel multifunctional bioactive materials for functional skincare and medical aesthetic applications.
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