[P002] Poster

1:00 PM - 1:50 PM JST(4:00 AM - 4:50 AM UTC)

[P01-074] Growth-Coupled CO₂ Feeding Strategy for Succinic Acid Production and Its Potential as a Biodegradable Polymer Precussor

○WOOSHIK SHIN¹, Jimin Hong^1,2, Byeonghwa Lee^1,3, Ki-Seob Hwang¹, Jaehoon Cho¹ (1. KITECH(Korea Institute of Industrial Technology) (Korea), 2. Sun Moon University (Korea), 3. Korea University (Korea))

Keywords:

Actinobacillus succinogenes,Succinic acid,Carbon dioxide feeding,Biodegradable polymer precursor

Actinobacillus succinogenes is a capnophilic, rumen derived bacterium capable of incorporating CO₂ into the biosynthesis of succinic acid, a key C4 dicarboxylic acid in central metabolism. Succinic acid has attracted significant attention not only as a platform chemical for industrial applications such as 1,4-butanediol and tetrahydrofuran, but also as a renewable precursor for biodegradable polymers, including poly(butylene succinate) (PBS) and related materials. In this study, we developed a refined CO₂ feeding strategy to enhance succinic acid production by regulating CO₂ availability through controlled supplementation of magnesium carbonate (MgCO₃ ). MgCO₃ serves as both a buffering agent and a slow-releasing CO₂ donor; however, its low solubility and high initial loading in conventional batch processes limit its efficiency and increase operational costs. To address these limitations, a growth-associated fed-batch approach was implemented, in which MgCO₃ was incrementally supplied according to cellular growth and metabolic activity. This strategy enabled more effective CO₂ utilization during active fermentation phases, leading to increased succinic acid productivity. At the same time, the total consumption of MgCO₃ was reduced, indicating improved resource efficiency. Notably, the enhanced production of bio-based succinic acid supports its role as a sustainable building block for biodegradable polymer synthesis, contributing to the development of environmentally friendly materials and a circular bioeconomy. Overall, this study demonstrates that growth-coupled CO₂ feeding is an effective strategy for improving fermentation performance while reinforcing the industrial relevance of succinic acid as a renewable and biodegradable polymer precursor.