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[P01-098] Effect of carbon sources on bacterial cellulose production by Komagataeibacter sp. BCSKY1001 isolated from pineapple vinegar

○Hyerim Son¹, Hong Ki Kim¹, Yunseok Song¹, Jungho Lee¹, Seunghee Kim¹, Suhyeon Kim¹, Hah Young Yoo¹ (1. Department of Biotechnology, Sangmyung University, Seoul 03016, Republic of Korea (Korea))

Keywords:

Bacterial cellulose,Pineapple vinegar,Komagataeibacter

Bacterial cellulose (BC) is a microbially produced cellulose biopolymer that shows high crystallinity, biocompatibility, and biodegradability, which supports its use in food, cosmetic, and biomedical materials. In this study, a BC-producing bacterium was isolated from pineapple vinegar and designated BCSKY1001. For taxonomic identification, the 16S rRNA sequencing was analyzed, and BCSKY1001 showed 99.78% sequence identity to Komagataeibacter melomenusus. To investigate the effect of the carbon source on BC production of BCSKY1001, eight carbon sources that are commonly applied in fermentation, including glucose, galactose, arabinose, fructose, lactose, sucrose, glycerol, and sorbitol, were used in BC production. Fermentation for producing BC was performed for 7 days, and each carbon source was supplied at 20 g/L. BC produced under each condition was purified and quantified on a dry-weight basis. As a result, BC production by BCSKY1001 was found to be 3.31, 0.47, 0.49, 3.61, 0.47, 1.29, 4.81, and 0.71g/L from the glucose, galactose, arabinose, fructose, lactose, sucrose, glycerol, and sorbitol medium, respectively. The highest BC production was observed with glycerol, reaching 4.81 g/L, which confirmed that glycerol is the most suitable carbon source for BC production using the BCSKY1001. In future work, fermentation variables such as substrate concentration, incubation time, and temperature will be optimized to maximize BC production from BCSKY1001.
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