[P002] Poster

1:00 PM - 1:50 PM JST(4:00 AM - 4:50 AM UTC)

[P01-120] Continuous electrochemical monitoring and imaging of cell aggregates and organ models

○Hitoshi Shiku¹ (1. Graduate School of Engineering, Tohoku University (Japan))

Keywords:

three dimensinal culture,organ on a chip,metabolic activity,enzymatic activity,electrochemiluminescence,electrochemcal sensor

[Purpose]Minimally invasive electrochemical measurement methods have been applied for continuous or long-term evaluation of the cell aggregates and organ models.

[Method]Microelectrode probes or electrochemical and electrochemiluminescence (ECL) devices have been developed to evaluate oxygen, reactive oxygen species, and enzyme activity.

[Results]We have succeeded to evaluate the changes in metabolic/enzyme activities over the cultivation dates during the differentiation process of stem cells, or co-culture spheroids with different cell types, the interface between different organs.

[Consideration]Fluctuations in current values during electrochemical monitoring are a serious problem in evaluation in cell culture medium and/or in vivo. Normalization of current values to the current in bulk, prevention of the electrode surface fouling can improve the quality of electrochemical imaging data.

[Conclusion]Further improvements are needed for electrochemical measurement methods to be widely used in actual diagnostic and regenerative medicine