Presentation Information
[P01-132]Development of LC-MS/MS-Based Simultaneous Analysis Method
for Psychoactive Drugs in Veterinary Forensic Analysis
○JeongWoo Kang1, Jae Won Byun1, HyunYoung Chae1, Bok-Kyung Ku1 (1. Animal and Plant Quarantine Agency (APQA) (Korea))
Keywords:
LC-MS/MS,Spectometry,Veterinary forensic,Narcotics,Psychotropics
Recent veterinary forensic analysis have attracted increasing attention and are being performed more actively, and reported misuse cases (e.g., unauthorized prescription of CNS acting drugs) underscore the need for rapid and reliable toxicological testing in animal-related investigations. In forensic practice, expanding use of psychotropic/CNS-active medications has increased demand for accurate multi-analyte screening and quantification methods1). Here, a rapid and efficient analytical workflow was established for the simultaneous detection of 26 central nervous system (CNS) drugs in veterinary forensic samples. Among the target analytes, 25 drugs were analyzed simultaneously using liquid chromatography&tandem mass spectrometry (LC&MS/MS), while propofol was analyzed separately using gas chromatography&tandem mass spectrometry (GC&MS/MS). LC&MS/MS conditions were
optimized using a Kinetex C18 column (50 × 3.0 mm, 2.6 µm) with gradient elution of 5 mM ammonium acetate/0.1% formic acid in water and 0.1% formic acid in methanol at a flow rate of 0.3 mL/min. All 25 drugs were separated and detected within a single 20 min run in multiple reaction monitoring (MRM) mode, with retention times of 1.52&15.08 min. Sample preparation employed a QuEChERS-based approach (extraction with 1% acetic acid in acetonitrile, followed by cleanup with magnesium sulfate and C18 sorbent, and PVDF filtration), leveraging the practicality of QuEChERS workflows reported for LC&MS/MS forensic applications2). Method validation was conducted in accordance with Commission Decision 2002/657/EC principles commonly applied to confirmatory LC&MS/MS methods3). The method showed excellent linearity (R² > 0.99) for all analytes, with recoveries of 73.16&89.90% and precision (RSD) below 10%. Limits of detection (LOD) ranged from 1.81 to 3.04 ng/mL and limits of quantification (LOQ) ranged from 6.02 to 10.14 ng/mL, with high selectivity and no interference observed in blank matrices. Overall, this validated simultaneous multi-drug method provides a practical tool for rapid screening and confirmation of CNS drug intoxication in animal abuse investigations and veterinary forensic toxicology, improving diagnostic efficiency compared with conventional single-analyte approaches
optimized using a Kinetex C18 column (50 × 3.0 mm, 2.6 µm) with gradient elution of 5 mM ammonium acetate/0.1% formic acid in water and 0.1% formic acid in methanol at a flow rate of 0.3 mL/min. All 25 drugs were separated and detected within a single 20 min run in multiple reaction monitoring (MRM) mode, with retention times of 1.52&15.08 min. Sample preparation employed a QuEChERS-based approach (extraction with 1% acetic acid in acetonitrile, followed by cleanup with magnesium sulfate and C18 sorbent, and PVDF filtration), leveraging the practicality of QuEChERS workflows reported for LC&MS/MS forensic applications2). Method validation was conducted in accordance with Commission Decision 2002/657/EC principles commonly applied to confirmatory LC&MS/MS methods3). The method showed excellent linearity (R² > 0.99) for all analytes, with recoveries of 73.16&89.90% and precision (RSD) below 10%. Limits of detection (LOD) ranged from 1.81 to 3.04 ng/mL and limits of quantification (LOQ) ranged from 6.02 to 10.14 ng/mL, with high selectivity and no interference observed in blank matrices. Overall, this validated simultaneous multi-drug method provides a practical tool for rapid screening and confirmation of CNS drug intoxication in animal abuse investigations and veterinary forensic toxicology, improving diagnostic efficiency compared with conventional single-analyte approaches
Comment
To browse or post comments, you must log in.Log in