Presentation Information

[P01-142]Sorting-free enrichment of biallelic two-copy recombinase-mediated cassette exchange (RMCE) integrants in GS-KO CHO cells via SiMPl-GS

○JaeJun Lee1, Chansik Yoon2, Yujin Kim3, Gyun Min Lee1,3 (1. Department of Biological Sciences, KAIST, Daejeon, Republic of Korea (Korea), 2. Center for Cellular Therapies and Cancer Immunology, The University of Texas Southwestern Medical Center, Dallas, TX (USA), 3. Graduate School of Engineering Biology, KAIST, Daejeon, Republic of Korea (Korea))

Keywords:

Site-specific integration (SSI),Recombinase-mediated cassette exchange (RMCE),GS-KO CHO cells,SiMPl-GS (split-intein GS),Biallelic two-copy integration

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