[P004] Poster

1:00 PM - 1:50 PM JST(4:00 AM - 4:50 AM UTC)

[P02-186] Adaptive laboratory evolution to shorten the lag phase of methanol-auxotrophic Escherichia coli

○Ying-Hsuan Lin¹, Yung-Wen Yang¹, Paul P. Lin¹ (1. Nation Yang Ming Chiao Tung University (Taiwan))

Keywords:

Adaptive Laboratory Evolution(ALE),Methanol Auxotrophic E. coli,Methanol Utilization

As the global community pursues the "Net-Zero 2050" goal, decarbonizing the chemical industry has become a top priority. As methanol can be produced from either CO2 or methane, methanol economy can play an important role in combating climate change. While using synthetic Escherichia coli to convert methanol into high-value chemicals is a promising solution, these strains often face a major bottleneck: suboptimal growth and long lag phases. Recent studies suggest that this is mainly caused by the accumulation of formaldehyde, which leads to toxic DNA-protein crosslinking (DPC).

Our preliminary findings show that the supplementation of five specific amino acids (Ile, Leu, Met, Ser, and Thr) significantly reduces the lag phase.Based on these results, we utilize adaptive laboratory evolution (ALE) while simultaneously implementing a stepwise elimination of these supplements to enhance the growth performance of a methanol-auxotrophic E. coli strain. Moving forward, we will perform whole-genome sequencing to compare the evolved strain with the parental genotype. Our objective is to identify the critical mutations responsible for these phenotypic improvements.